Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,974,914	Δ1 bp	coding (628/1419 nt)	araE ←	arabinose‑proton symporter AraE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,974,914	0	G	.	100.0%	169.5 / NA	39	coding (628/1419 nt)	araE	arabinose‑proton symporter AraE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/16);  total (23/16)

GTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974780‑2975047                                                                                                                                       |                                                                                                                                      gTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACcccct                                                                                                                                   >  2:357056/1‑138 (MQ=255)          gATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCgc                                                                                                                           >  2:81626/1‑137 (MQ=255)          gATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCgc                                                                                                                           >  2:107612/1‑137 (MQ=255)                 aaCAGTGCCCAACCGGCCTGTTTTAACTTCAGGCTTTCCCGAATTTCGTTGAATTTTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATTCTTCTTCCGCCTCAATATGAC‑CTCCTTTTCCGCcagccagc                                                                                                                   <  1:536811/138‑1 (MQ=255)                 aaCAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAgcg                                                                                                                  <  1:107612/139‑1 (MQ=255)                 aaCAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAgcg                                                                                                                  <  1:81626/139‑1 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAgcg                                                                                                                  <  2:564188/134‑1 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAgcg                                                                                                                  >  1:564188/1‑134 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:262615/1‑139 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:548120/1‑139 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:466320/1‑139 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  2:306492/1‑139 (MQ=255)                      tGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGc                                                                                                             >  1:521710/1‑139 (MQ=255)                                     ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGa                                                                                              >  1:345923/1‑139 (MQ=255)                                     ttttAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGa                                                                                              >  1:227424/1‑139 (MQ=255)                                               aGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaa                                                                                     >  2:429874/1‑138 (MQ=255)                                                ggCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGaata                                                                                   <  1:429874/139‑1 (MQ=255)                                                                              gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCa                                                                                                    <  1:252762/92‑1 (MQ=255)                                                                              gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCa                                                                                                    >  2:252762/1‑92 (MQ=255)                                                                              gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGa                                                        <  1:408050/136‑1 (MQ=255)                                                                              gcgcTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAAcc                                                     >  1:318419/1‑139 (MQ=255)                                                                                                 gcgcAGCATACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGt                                  <  2:318419/139‑1 (MQ=255)                                                                                                    cagcaTACGCAATACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               <  2:345923/139‑1 (MQ=255)                                                                                                              aaTACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACt                     <  1:565642/139‑1 (MQ=255)                                                                                                               aTACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          >  2:63437/1‑101 (MQ=255)                                                                                                               aTACTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCaa                                                          <  1:63437/101‑1 (MQ=255)                                                                                                                 aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               >  1:484854/1‑126 (MQ=255)                                                                                                                 aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTAc                               <  2:484854/126‑1 (MQ=255)                                                                                                                 aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  1:332390/1‑139 (MQ=255)                                                                                                                 aCTTCTTCCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGaa                  >  1:150908/1‑139 (MQ=255)                                                                                                                     cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTGTTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACg                <  2:287419/137‑1 (MQ=255)                                                                                                                     cttcCGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCt              >  1:434834/1‑139 (MQ=255)                                                                                                                        ccGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTa           >  1:98012/1‑139 (MQ=255)                                                                                                                        ccGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTa           >  1:352573/1‑139 (MQ=255)                                                                                                                         cGCCTCAATATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTa           <  1:250841/138‑1 (MQ=255)                                                                                                                               aaTATGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGAt     >  1:107608/1‑138 (MQ=255)                                                                                                                                atatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATa    >  2:479872/1‑138 (MQ=255)                                                                                                                                atatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATa    >  1:392422/1‑138 (MQ=255)                                                                                                                                 tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  2:332390/139‑1 (MQ=255)                                                                                                                                 tatGAC‑CCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATaaa  <  2:150908/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTTAACTTCAGGCTTTCACGAATTTCGTTGAGTTCTTCTCGCGCTTTTTCCGACGTATCGCGCAGCATACGCAATACTTCTTCCGCCTCAATATGACGCCCCTTTTCCGCCAGCCAGCGCGGGCTATTTGGCAGGAAGACTACCAGAATAATCAGCAGAACTGCTGGTAAAGCAAGAACCCCCAACATTGCGCGCCAGTTACCGCTATAACTGAACGCTGTATCGGATAAA  >  NZ_CP009273/2974780‑2975047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑