Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,000,345	(A)5→4	coding (722/1188 nt)	ygeW →	knotted carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,000,341	0	A	.	100.0%	158.3 / NA	36	coding (718/1188 nt)	ygeW	knotted carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/14);  total (22/14)

CTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTG  >  NZ_CP009273/3000211‑3000467                                                                                                                                   |                                                                                                                               ctctCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATg                                                                                                                       >  1:380049/1‑139 (MQ=255)             tGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCg                                                                                                           >  1:179004/1‑139 (MQ=255)              ggCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCgg                                                                                                          >  2:56313/1‑139 (MQ=255)              ggCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCgg                                                                                                          >  2:178978/1‑139 (MQ=255)              ggCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCgg                                                                                                          >  2:12947/1‑139 (MQ=255)                         ctctGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTcc                                                                                               >  1:36659/1‑139 (MQ=255)                              tACCTCAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             <  1:533955/136‑1 (MQ=255)                              tACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaaca                                                                                                                           >  1:168385/1‑105 (MQ=255)                              tACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGaaaaca                                                                                                                           <  2:168385/107‑3 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTa                                                                                                                    <  1:504154/105‑1 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTa                                                                                                                    >  2:504154/1‑105 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             <  2:345891/128‑1 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             >  1:345891/1‑128 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  2:543854/1‑139 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  >  1:438908/1‑139 (MQ=255)                                      ggCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAg                                                                                  <  1:543854/139‑1 (MQ=255)                                        catcatCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCa                                                                                <  1:286556/139‑1 (MQ=255)                                                            gCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAg                                                              <  2:36659/137‑1 (MQ=255)                                                                        aTGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             <  2:66695/94‑1 (MQ=255)                                                                        aTGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGt                                                                                             >  1:66695/1‑94 (MQ=255)                                                                        aTGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGtt                                                >  2:556077/1‑139 (MQ=255)                                                                             aCCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATcc                                           >  2:196451/1‑139 (MQ=255)                                                                             aCCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATcc                                           >  2:506838/1‑139 (MQ=255)                                                                             aCCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATcc                                           >  1:291490/1‑139 (MQ=255)                                                                                  ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGc                                                          >  2:426844/1‑119 (MQ=255)                                                                                  ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGc                                                          <  1:426844/119‑1 (MQ=255)                                                                                  ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGt                                      >  1:218639/1‑139 (MQ=255)                                                                                  ggCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGt                                      >  1:391041/1‑139 (MQ=255)                                                                                         ccGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTc                                                                                                             <  2:134995/61‑1 (MQ=255)                                                                                         ccGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTc                                                                                                             >  1:134995/1‑61 (MQ=255)                                                                                          cGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGc                               <  1:295404/138‑1 (MQ=255)                                                                                                    cGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACaa                      <  2:218639/137‑1 (MQ=255)                                                                                                    cGACCTGATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAg                    <  1:506838/139‑1 (MQ=255)                                                                                                          gATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGa                                                       <  2:279483/98‑1 (MQ=255)                                                                                                          gATCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGa                                                       >  1:279483/1‑98 (MQ=255)                                                                                                           aTCCCGGATGTGGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATgg              >  2:478431/1‑138 (MQ=255)                                                                                                                   tgtgGTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTa     <  2:441878/139‑1 (MQ=255)                                                                                                                       gTTGAAGTGGCG‑AAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTg  >  2:429952/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                               CTCTCCAAGCTATGGCAAACCGCTCTCTGTACCACAAGGCATCATCGGTCTGATGACTCGCTTCGGTATGGATGTCACCCTGGCCCATCCGGAAGGCTACGACCTGATCCCGGATGTGGTTGAAGTGGCGAAAAACAATGCTAAAGCCTCCGGTGGTAGCTTCCGTCAGGTCACCAGCATGGAAGAAGCCTTCAAAGACGCAGACATCGTTTATCCGAAGTCATGGGCACCTTACAAAGTGATGGAAGAGCGTACTG  >  NZ_CP009273/3000211‑3000467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑