| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,238,476 | 0 | G | A | 57.1% | 5.1 / 12.9 | 14 | R21C (CGT→TGT) | ldcA | muramoyltetrapeptide carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base A (3/5); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGC > NZ_CP009273/1238345‑1238609 | agtcagtcTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg > 1:193683/1‑139 (MQ=255) tcTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGc > 2:279592/1‑139 (MQ=255) cAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGtt > 1:128155/1‑138 (MQ=255) ggAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGTTTAATg < 1:340127/139‑1 (MQ=255) cGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCaa < 2:120643/139‑1 (MQ=255) tctGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTa < 1:8345/122‑1 (MQ=255) tctGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTa > 2:8345/1‑122 (MQ=255) cTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTaa < 1:508497/117‑1 (MQ=255) cTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTaa > 2:508497/1‑117 (MQ=255) cTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAAtt > 2:402873/1‑139 (MQ=255) cgacgGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCt < 2:461937/139‑1 (MQ=255) gACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa < 1:402873/137‑1 (MQ=255) gACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACACAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa < 2:128155/137‑1 (MQ=255) ccACACAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGc < 1:279592/138‑1 (MQ=255) | AGTCAGTCTGGCAAGGGAATTGAGATCTTCCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGC > NZ_CP009273/1238345‑1238609 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |