Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,121,8600AG59.1% 4.0 / 27.2 22N170N (AAT→AAC) wcaFcolanic acid biosynthesis acetyltransferase WcaF
Reads supporting (aligned to +/- strand):  ref base A (5/4);  new base G (7/6);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCA  >  NZ_CP009273/2121765‑2121993
                                                                                               |                                                                                                                                     
ttACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAacac                                                                                            >  2:562023/1‑139 (MQ=255)
          gatgaGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTg                                                                                   >  2:552367/1‑138 (MQ=255)
                agCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCac                                                                            >  2:182870/1‑139 (MQ=255)
                  cGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCa                                                                             >  1:101361/1‑136 (MQ=255)
                  cGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCa                                                                             <  2:101361/136‑1 (MQ=255)
                     cTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACgg                                                                       <  1:554834/139‑1 (MQ=255)
                                tattatTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGcc                                                             >  1:86211/1‑138 (MQ=255)
                                     ttCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATtgt                                                       <  1:562023/139‑1 (MQ=255)
                                          ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCa                                                                                 >  1:15965/1‑108 (MQ=255)
                                          ctGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCa                                                                                 <  2:15965/108‑1 (MQ=255)
                                           tGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTaa                                                                                                  <  1:457024/90‑1 (MQ=255)
                                           tGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTaa                                                                                                  >  2:457024/1‑90 (MQ=255)
                                                                     aCGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGt                                                                                    <  1:514210/78‑1 (MQ=255)
                                                                     aCGCGTTCGCGTATCACCACTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGt                                                                                    >  2:514210/1‑78 (MQ=255)
                                                                                    accacTGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCg         <  1:552367/138‑1 (MQ=255)
                                                                                        cTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAAt    <  1:197614/139‑1 (MQ=255)
                                                                                         tGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATtgt                                                       <  2:223830/87‑1 (MQ=255)
                                                                                         tGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATtgt                                                       >  1:223830/1‑87 (MQ=255)
                                                                                         tGCGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAAt    >  1:424766/1‑138 (MQ=255)
                                                                                           cGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGcc       <  1:86010/133‑1 (MQ=255)
                                                                                           cGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGcc       >  2:86010/1‑133 (MQ=255)
                                                                                           cGGGGTTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATca  >  2:22609/1‑138 (MQ=255)
                                                                                               |                                                                                                                                     
TTACACCGGTGATGAGAGCGACTTTTGACATGTATTATTCCTCTGTATTTTTGAATTTATTCAGTTTCAACGCGTTCGCGTATCACCACTGCGGGATTCCCCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCA  >  NZ_CP009273/2121765‑2121993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑