| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,544,265 | 0 | A | T | 70.0% | 28.8 / 17.0 | 20 | I104N (ATC→AAC) | yfeY | RpoE‑regulated lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base T (9/5); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCA > NZ_CP009273/2544142‑2544381 | ttATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGc < 2:158390/139‑1 (MQ=255) atcaCCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGtt > 2:520721/1‑138 (MQ=255) cATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCAc > 2:201308/1‑139 (MQ=255) aTGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTtcat < 1:296373/139‑1 (MQ=255) aTGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTtcat < 2:492683/139‑1 (MQ=255) cGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcac > 1:178441/1‑139 (MQ=255) gCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcac > 2:199828/1‑138 (MQ=255) gCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcac > 2:135297/1‑138 (MQ=255) tAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGcaccac > 1:265703/1‑139 (MQ=255) ggTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGAt > 1:18856/1‑76 (MQ=255) ggTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGAt < 2:18856/76‑1 (MQ=255) ttttAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt < 2:178441/139‑1 (MQ=255) agcagGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTgcgc < 2:265703/139‑1 (MQ=255) agGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAgg > 1:268550/1‑139 (MQ=255) aTCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCa > 2:467510/1‑139 (MQ=255) aTCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCa > 1:224589/1‑139 (MQ=255) tCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt < 1:566978/61‑1 (MQ=255) tCCAGCACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt > 2:566978/1‑61 (MQ=255) cACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt > 1:326616/1‑102 (MQ=255) cACGTCAATGCGACTGTTCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt < 2:326616/102‑1 (MQ=255) | TTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCA > NZ_CP009273/2544142‑2544381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |