| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,376,030 | 0 | A | G | 61.5% | 14.3 / 27.3 | 26 | I158V (ATC→GTC) | degS | outer membrane‑stress sensor serine endopeptidase DegS |
| Reads supporting (aligned to +/- strand): ref base A (4/6); new base G (5/11); total (9/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCATTGCTGGTGGGATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTCCATTAAC > NZ_CP009273/3375902‑3376149 | aaGCATTGCTGGTGGGATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAATTTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAAc < 1:211020/138‑1 (MQ=255) cATTGCTGGTGGGATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTa < 1:489726/139‑1 (MQ=255) ggATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCggg < 2:260814/138‑1 (MQ=255) gATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTTCCTACCATCCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCa < 1:38803/139‑1 (MQ=255) aCTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCa < 2:509740/138‑1 (MQ=255) tAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTAcc < 1:87345/137‑1 (MQ=255) tAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTAcc < 1:431611/137‑1 (MQ=255) cTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTTGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGatta > 2:279322/1‑139 (MQ=255) cTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTAACTAACATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGGAGGACTGGCGGGCGGGAAACCCTAAAAACCCCGGGAGACCACTACCCCGGGGgtta > 1:305139/1‑139 (MQ=255) gTACTTAAAATTAATGCCACTGGCGGTTTACCCACCATTTCAA‑TTTATGCACGTCGCGGACCGCACATTGGCGACGGAGTACTGGCGAACGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGAGTATTAggg > 2:98455/1‑137 (MQ=255) aaaTTAATGCCACTGGCGGTTTACCTACCATTTCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTa > 1:397803/1‑94 (MQ=255) aaaTTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTa < 2:397803/94‑1 (MQ=255) aaaTTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGTCCATTACCCAGGTGATTATTAGTGCCActtg < 2:261947/139‑4 (MQ=255) aTGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGCTCGAAt < 2:561981/139‑1 (MQ=255) aTGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt < 1:25956/139‑1 (MQ=255) cACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGt < 1:279322/139‑1 (MQ=255) tCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt < 1:268962/114‑1 (MQ=255) tCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt > 2:268962/1‑114 (MQ=255) tACCGCACATTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGc < 2:186778/139‑1 (MQ=255) cacaTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATg < 1:330119/133‑1 (MQ=255) cacaTTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATg > 2:330119/1‑133 (MQ=255) aTTGGCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTCCAtt > 1:533764/1‑138 (MQ=255) tGGCGACGTAGTACTGGCGGTCGGGAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAAACGATGCTTCCATTAAc > 1:27845/1‑139 (MQ=255) ggCGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTCCATTAAc < 2:220407/138‑1 (MQ=255) cGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt > 2:194051/1‑81 (MQ=255) cGACGTAGTACTGGCGGTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt < 1:194051/81‑1 (MQ=255) aTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt < 2:412050/65‑1 (MQ=255) aTCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAAt > 1:412050/1‑65 (MQ=255) | AAGCATTGCTGGTGGGATCTGACTCTCTAACCGATCTGGCGGTACTTAAAATTAATGCCACTGGCGGTTTACCTACCATTCCAA‑TTAATGCACGTCGCGTACCGCACATTGGCGACGTAGTACTGGCGATCGGTAACCCGTACAACCTCGGGCAGACCATTACCCAGGGGATTATTAGTGCCACGGGTCGAATCGGTCTGAACCCGACCGGGCGGCAAAACTTCCTCCAAACCGATGCTTCCATTAAC > NZ_CP009273/3375902‑3376149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |