Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,680,7300AG62.7% 35.8 / 60.6 51S602P (TCC→CCC) bcsCcellulose synthase complex outer membrane protein BcsC
Reads supporting (aligned to +/- strand):  ref base A (11/8);  new base G (15/17);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TATCCACTTCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCACTGCGCAC  >  NZ_CP009273/3680592‑3680864
                                                                                                                                          |                                                                                                                                      
tatCCACTTCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGa                                                                                                                                        <  1:49136/139‑1 (MQ=255)
  tCCACTTCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAg                                                                                                                                      <  1:139140/139‑1 (MQ=255)
  tCCACTTCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAg                                                                                                                                      >  1:169901/1‑139 (MQ=255)
       ttCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAggtggt                                                                                                                                 >  1:142194/1‑139 (MQ=255)
       ttCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAggtggt                                                                                                                                 >  1:86290/1‑139 (MQ=255)
            tCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTg                                                                                                                            >  1:44687/1‑139 (MQ=255)
                aCCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGgcgc                                                                                                                        <  2:142194/139‑1 (MQ=255)
                   aaGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCgcagc                                                                                                                     <  2:371791/139‑1 (MQ=255)
                           cGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATcgcttc                                                                                                             >  2:365501/1‑139 (MQ=255)
                                                    cAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTtct                                                                                    >  1:103745/1‑139 (MQ=255)
                                                     aGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTtctc                                                                                   >  2:195048/1‑139 (MQ=255)
                                                       gACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGc                                                                                 <  2:121936/139‑1 (MQ=255)
                                                         cATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAg                                                                               <  2:77686/139‑1 (MQ=255)
                                                         cATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAg                                                                               <  2:20449/139‑1 (MQ=255)
                                                            tCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCg                                                                            >  1:331437/1‑139 (MQ=255)
                                                            tCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCg                                                                            >  2:339729/1‑139 (MQ=255)
                                                                 tatGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAg                                                                       <  2:331437/139‑1 (MQ=255)
                                                                 tatGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAg                                                                       >  1:509579/1‑139 (MQ=255)
                                                                   tGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     >  1:29278/1‑139 (MQ=255)
                                                                   tGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     <  1:61685/139‑1 (MQ=255)
                                                                      aGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCgggt                                                                  >  2:257157/1‑137 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCgg                                                                    >  2:460478/1‑135 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCgg                                                                    <  1:460478/135‑1 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTc                                                                >  2:4870/1‑139 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCAt                                                                                                                   >  1:471214/1‑88 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCAt                                                                                                                   <  2:471214/88‑1 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTc                                                                >  1:359851/1‑139 (MQ=255)
                                                                        cgcgGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTc                                                                >  2:21649/1‑139 (MQ=255)
                                                                          cgggcggCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTc                                                                >  1:440292/1‑137 (MQ=255)
                                                                                  ggTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGa                                                      <  1:195048/139‑1 (MQ=255)
                                                                                  ggTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGa                                                      <  1:21649/139‑1 (MQ=255)
                                                                                            cgtcgtTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCa                                            <  1:459250/139‑1 (MQ=255)
                                                                                              tcgtTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGt                                          >  1:429722/1‑139 (MQ=255)
                                                                                                       cgcCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAAcc                                 <  1:334138/139‑1 (MQ=255)
                                                                                                       cgcCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAAcc                                 <  2:314173/139‑1 (MQ=255)
                                                                                                       cgcCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAAc                                  <  1:260351/138‑1 (MQ=255)
                                                                                                         cccAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAg                               <  2:169901/139‑1 (MQ=255)
                                                                                                         cccAGTCAGCCAGCGTGAGGTCAATACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAg                               >  2:326345/1‑139 (MQ=255)
                                                                                                            aGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTc                            <  1:58810/139‑1 (MQ=255)
                                                                                                                 gccagcGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGaa                       >  1:39172/1‑139 (MQ=255)
                                                                                                                          aGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     >  1:528270/1‑84 (MQ=255)
                                                                                                                          aGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     <  2:528270/84‑1 (MQ=255)
                                                                                                                               aaTACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTTCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCAc         >  1:110734/1‑139 (MQ=255)
                                                                                                                                aTACGCGTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCACt        <  2:429722/139‑1 (MQ=255)
                                                                                                                                   cgcgTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCACTgcg     <  2:440292/139‑1 (MQ=255)
                                                                                                                                      gTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAg                                                                       >  2:29941/1‑70 (MQ=255)
                                                                                                                                      gTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAg                                                                       <  1:29941/70‑1 (MQ=255)
                                                                                                                                      gTGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCACTGCGCAc  <  2:29278/139‑1 (MQ=255)
                                                                                                                                       tGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     >  2:347920/1‑71 (MQ=255)
                                                                                                                                       tGGGAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCg                                                                     <  1:347920/71‑1 (MQ=255)
                                                                                                                                         gAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAAcc                                 >  1:65962/1‑105 (MQ=255)
                                                                                                                                         gAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAAcc                                 <  2:65962/105‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TATCCACTTCCGTCAGACCAAGAATGGCGTCGGCGTTAGCTGGCTCCCGCGTCAGGACATTCTGATATGCAGCGCGGGCGGCGGTGTAATCACGTCGTTGTTGCGCCCAGTCAGCCAGCGTGAGGTCAATACGCGTGGAAGGTGGTTGCTGGCGCAGCATCGCTTCCGCTTCTGCCTCTTTGCCGCTTTCTCGCAGGCGGTTAGCGGTTTCCAGCACCTGATCGCTTTGCAGTCGATTAACCAGCTCCTGAATATTGCTGTTCCACTGCGCAC  >  NZ_CP009273/3680592‑3680864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑