Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	10,865	A→C	G164G (GGT→GGG)	msyB ←	acidic protein MsyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	10,865	0	A	C	100.0%	110.2 / NA	32	G164G (GGT→GGG)	msyB	acidic protein MsyB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/13);  total (19/13)

CTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACG  >  NZ_CP009273/10732‑10990                                                                                                                                      |                                                                                                                              cTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAACGCAAATGCGACACCCCttt                                                                                                                          >  2:73048/1‑139 (MQ=255)      aCCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGcc                                                                                                                      <  1:344565/138‑1 (MQ=255)            tttAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAAtt                                                                                                               >  1:234953/1‑139 (MQ=255)                        ccTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTtcat                                                                                                     >  2:314301/1‑137 (MQ=255)                                 ccTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCgg                                                                                            >  1:236496/1‑137 (MQ=255)                                   tCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcag                                                                                         >  1:162247/1‑138 (MQ=255)                                   tCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcag                                                                                         >  1:295388/1‑138 (MQ=255)                                              cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                                             >  2:246169/1‑139 (MQ=255)                                                   cgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAt                                                                        >  1:534674/1‑139 (MQ=255)                                                    gcagcaAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                       <  2:60387/139‑1 (MQ=255)                                                    gcagcaAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                       <  1:246169/139‑1 (MQ=255)                                                    gcagcaAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                       <  2:162247/139‑1 (MQ=255)                                                        caAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                   <  2:234953/139‑1 (MQ=255)                                                                 cccAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCg                                                          <  1:437973/139‑1 (MQ=255)                                                                            tCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTg                                                  >  1:565986/1‑136 (MQ=255)                                                                              aTTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGa                                              >  1:275357/1‑138 (MQ=255)                                                                                tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                                             >  1:6954/1‑105 (MQ=255)                                                                                tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAg                                                                             <  2:6954/105‑1 (MQ=255)                                                                                tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAAc                                           >  2:457106/1‑139 (MQ=255)                                                                                     cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                                        <  1:354990/137‑1 (MQ=255)                                                                                     cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                                        >  2:354990/1‑137 (MQ=255)                                                                                        gggTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCt                                    >  1:25057/1‑138 (MQ=255)                                                                                          gTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTAt                                 <  2:275357/139‑1 (MQ=255)                                                                                            gCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGt                               >  2:488253/1‑139 (MQ=255)                                                                                                    tGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                   <  2:310355/95‑1 (MQ=255)                                                                                                    tGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCAcc                                                                   >  1:310355/1‑95 (MQ=255)                                                                                                        ggTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCtt                                                                                 <  2:271860/77‑1 (MQ=255)                                                                                                        ggTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCtt                                                                                 >  1:271860/1‑77 (MQ=255)                                                                                                         gTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGcagcag                                                                                      <  2:72777/71‑1 (MQ=255)                                                                                                         gTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGcagcag                                                                                      >  1:72777/1‑71 (MQ=255)                                                                                                            aGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTttct               >  2:522263/1‑139 (MQ=255)                                                                                                                         gCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACg  <  2:295388/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                              CTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGGTATTACG  >  NZ_CP009273/10732‑10990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑