Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,246,701	A→G	I291I (ATT→ATC)	yhaJ ←	DNA‑binding transcriptional regulator YhaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,246,701	0	A	G	100.0%	47.7 / NA	14	I291I (ATT→ATC)	yhaJ	DNA‑binding transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/9);  total (5/9)

CAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAA  >  NZ_CP009273/3246584‑3246802                                                                                                                      |                                                                                                      cagaaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAt                                                                                  <  2:392975/139‑1 (MQ=255)      aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                             <  1:123112/139‑1 (MQ=255)                          aaGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                             >  1:501994/1‑119 (MQ=255)                          aaGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                             <  2:501994/119‑1 (MQ=255)                            gTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGc                                                       <  1:151001/139‑1 (MQ=255)                                      aaTTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataa                                             <  2:418635/139‑1 (MQ=255)                                                    ttGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCg                               <  2:488635/139‑1 (MQ=255)                                                       gggCAATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTg                            >  1:78317/1‑139 (MQ=255)                                                                 tCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                  >  1:237084/1‑107 (MQ=255)                                                                 tCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                  <  2:237084/107‑1 (MQ=255)                                                                       cTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTcccc                                                                       <  1:92613/80‑1 (MQ=255)                                                                       cTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTcccc                                                                       >  2:92613/1‑80 (MQ=255)                                                                                aaCAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGc    <  2:78317/138‑1 (MQ=255)                                                                                 aCAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCaa  >  1:374375/1‑139 (MQ=255)                                                                                                                      |                                                                                                      CAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAA  >  NZ_CP009273/3246584‑3246802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑