Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,332,009	A→G	V200A (GTC→GCC)	mlaE ←	lipid asymmetry maintenance ABC transporter permease subunit MlaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,332,009	0	A	G	100.0%	76.4 / NA	21	V200A (GTC→GCC)	mlaE	lipid asymmetry maintenance ABC transporter permease subunit MlaE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTGG  >  NZ_CP009273/3331874‑3332142                                                                                                                                        |                                                                                                                                      gCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCa                                                                                                                                    <  2:378717/139‑1 (MQ=255)  ccAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAg                                                                                                                                   <  1:355354/139‑1 (MQ=255)  ccAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAg                                                                                                                                   <  2:173580/139‑1 (MQ=255)       gaCGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCa                                                                                                                              <  1:482824/139‑1 (MQ=255)                       tGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACgg                                                                                                               >  1:299736/1‑138 (MQ=255)                               gTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCa                                                                                                      >  2:398537/1‑139 (MQ=255)                                                       gTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGcc                                                                                                 <  1:265977/120‑1 (MQ=255)                                                       gTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGcc                                                                                                 >  2:265977/1‑120 (MQ=255)                                                                 cgtcgtAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCttt                                                                    >  2:558865/1‑139 (MQ=255)                                                                                 caacGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACg                                                          <  1:568899/133‑1 (MQ=255)                                                                                        aTCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc                                             <  1:558865/139‑1 (MQ=255)                                                                                              gTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc                                             <  1:490/133‑1 (MQ=255)                                                                                              gTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc                                             >  2:490/1‑133 (MQ=255)                                                                                                 aCCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAg                                     <  1:415670/138‑1 (MQ=255)                                                                                                   cGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATc                                  >  2:9636/1‑139 (MQ=255)                                                                                                               caccacGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg                                                                                  >  1:435683/1‑79 (MQ=255)                                                                                                               caccacGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg                                                                                  <  2:435683/79‑1 (MQ=255)                                                                                                                   acGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGaaaa                  <  1:9636/139‑1 (MQ=255)                                                                                                                                   aGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTgg  >  1:135861/1‑139 (MQ=255)                                                                                                                                     ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGaaaa                  >  1:97692/1‑121 (MQ=255)                                                                                                                                     ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGaaaa                  <  2:97692/121‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAATAACCGTCAACAGTGG  >  NZ_CP009273/3331874‑3332142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑