Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,351,627	C→T	L1182L (CTG→TTG)	gltB →	glutamate synthase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,351,627	0	C	T	100.0%	75.1 / NA	20	L1182L (CTG→TTG)	gltB	glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/10);  total (10/10)

TGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCA  >  NZ_CP009273/3351503‑3351730                                                                                                                             |                                                                                                        tGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGa                                                                                             <  2:122920/137‑1 (MQ=255)                       cgcgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGa                                                                                             <  1:22333/115‑1 (MQ=255)                       cgcgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGa                                                                                             >  2:22333/1‑115 (MQ=255)                             cTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAg                                                               >  2:461561/1‑139 (MQ=255)                                aTGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAgg                                                              <  2:42786/137‑1 (MQ=255)                                   gCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAgg                                                         <  2:168859/139‑1 (MQ=255)                                     acaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                       <  2:466400/139‑1 (MQ=255)                                                        cTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTa                                                            >  1:31118/1‑115 (MQ=255)                                                        cTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTa                                                            <  2:31118/115‑1 (MQ=255)                                                                 cTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGt                           >  2:343292/1‑139 (MQ=255)                                                                        gTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                       >  2:114747/1‑104 (MQ=255)                                                                        gTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                       <  1:114747/104‑1 (MQ=255)                                                                         tCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGACAACAACCCGCCGTTTGATAAc                   >  2:212926/1‑139 (MQ=255)                                                                            cACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                       <  2:443875/100‑1 (MQ=255)                                                                            cACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCa                                                       >  1:443875/1‑100 (MQ=255)                                                                              ccGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCct              >  1:449202/1‑139 (MQ=255)                                                                                 aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGa                                                                                             >  1:52410/1‑57 (MQ=255)                                                                                 aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGa                                                                                             <  2:52410/57‑1 (MQ=255)                                                                                      ctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcg      >  1:426554/1‑139 (MQ=255)                                                                                          aaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGTTGGCGCTGTCGAAGCTGCGGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACGGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCa  <  2:426554/139‑1 (MQ=255)                                                                                                                             |                                                                                                        TGAGTTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCA  >  NZ_CP009273/3351503‑3351730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑