| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,473,413 | A→G | M185T (ATG→ACG) | kefB ← | glutathione‑regulated potassium‑efflux system protein KefB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,473,413 | 0 | A | G | 100.0% | 78.9 / NA | 23 | M185T (ATG→ACG) | kefB | glutathione‑regulated potassium‑efflux system protein KefB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/9); total (14/9) | |||||||||||
ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGC > NZ_CP009273/3473279‑3473526 | aTGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc < 2:92018/139‑1 (MQ=255) gCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCTCCAATCAACATGCCGACAAACGCCCGCACCTTCGTGCCGa > 2:387432/1‑139 (MQ=255) cGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGAc > 1:342259/1‑139 (MQ=255) gcagcagcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAACTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGGCGa > 1:233657/1‑138 (MQ=255) cagcagcGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGaaa > 2:440059/1‑139 (MQ=255) cggTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGa < 1:561325/139‑1 (MQ=255) aCGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGATCGTCTGCCGACCCCGCCAACAACGGCa > 1:535612/1‑139 (MQ=255) ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGc > 2:558775/1‑85 (MQ=255) ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGc < 1:558775/85‑1 (MQ=255) ccAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt > 2:392368/1‑138 (MQ=255) aaGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc > 1:101366/1‑139 (MQ=255) gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc > 1:180742/1‑87 (MQ=255) gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc < 2:180742/87‑1 (MQ=255) aaTAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc < 1:497002/85‑1 (MQ=255) aaTAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATc > 2:497002/1‑85 (MQ=255) tACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAGACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCg < 2:186065/79‑1 (MQ=255) tACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAGACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCg > 1:186065/1‑79 (MQ=255) ccGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa < 2:342259/138‑1 (MQ=255) ccGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa < 1:440059/138‑1 (MQ=255) gACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTc < 1:84221/82‑1 (MQ=255) gACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTc > 2:84221/1‑82 (MQ=255) tAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGaaa > 1:96659/1‑138 (MQ=255) cATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGc > 2:441556/1‑139 (MQ=255) | ATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGC > NZ_CP009273/3473279‑3473526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |