Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,747,593 C→G A66P (GCC→CCC)  yiaW ← DUF3302 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,747,5930CG100.0% 150.7 / NA 42A66P (GCC→CCC) yiaWDUF3302 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (21/21);  total (21/21)

CATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGA  >  NZ_CP009273/3747457‑3747724
                                                                                                                                        |                                                                                                                                    
cATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGgcc                                                                                                                                    <  2:270380/139‑1 (MQ=255)
cATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGgcc                                                                                                                                    <  1:342960/139‑1 (MQ=255)
  tAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCa                                                                                                                                   >  2:53930/1‑138 (MQ=255)
        ttACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCca                                                                                                                             >  1:499102/1‑138 (MQ=255)
        ttACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCAAAATCca                                                                                                                             >  1:161261/1‑138 (MQ=255)
                cAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg                                                                                                                    >  2:443388/1‑139 (MQ=255)
                cAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg                                                                                                                    >  2:135453/1‑139 (MQ=255)
                              aTGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt                                                                                                       <  2:172906/138‑1 (MQ=255)
                                         cTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGa                                                                                           <  1:53930/139‑1 (MQ=255)
                                                        ggCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  >  2:319811/1‑101 (MQ=255)
                                                        ggCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  <  1:319811/101‑1 (MQ=255)
                                                         gCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCaa                                                                           >  2:418242/1‑139 (MQ=255)
                                                           aaTGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACa                                                                         <  2:499102/139‑1 (MQ=255)
                                                               cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATgca                                                                     >  2:459805/1‑137 (MQ=255)
                                                               cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGaa                                                                     <  1:18990/139‑1 (MQ=255)
                                                                      gcagttgcaTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt                                                               <  2:298975/138‑1 (MQ=255)
                                                                                       gAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTAc                                             >  1:420877/1‑139 (MQ=255)
                                                                                               gCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑ttttttt                                    >  1:111661/1‑138 (MQ=255)
                                                                                                   tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  <  2:116217/58‑1 (MQ=255)
                                                                                                   tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  >  1:116217/1‑58 (MQ=255)
                                                                                                   tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑tttttttggc                                 >  1:411813/1‑134 (MQ=255)
                                                                                                   tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑tttttttgg                                  <  1:390455/137‑4 (MQ=255)
                                                                                                   tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCaa                                >  2:138676/1‑139 (MQ=255)
                                                                                                     cATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAAt                               <  2:411813/139‑1 (MQ=255)
                                                                                                         ccccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAgg                           <  2:420877/138‑1 (MQ=255)
                                                                                                         ccccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAgg                           <  1:190805/138‑1 (MQ=255)
                                                                                                          cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt                                                               <  2:118101/102‑1 (MQ=255)
                                                                                                          cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGt                                                               >  1:118101/1‑102 (MQ=255)
                                                                                                          cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGt                          <  1:476202/138‑1 (MQ=255)
                                                                                                           ccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAgg                           >  1:388962/1‑137 (MQ=255)
                                                                                                           ccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAgg                           <  2:388962/137‑1 (MQ=255)
                                                                                                               ccccGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAAt                               <  1:500127/129‑1 (MQ=255)
                                                                                                               ccccGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAAt                               >  2:500127/1‑129 (MQ=255)
                                                                                                                    cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt                                                                                                       >  1:99270/1‑52 (MQ=255)
                                                                                                                    cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt                                                                                                       <  2:99270/52‑1 (MQ=255)
                                                                                                                    cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAGGTAGGGAATATc                <  1:443388/139‑1 (MQ=255)
                                                                                                                    cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCa               >  1:528926/1‑139 (MQ=255)
                                                                                                                    cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCa               >  1:305292/1‑139 (MQ=255)
                                                                                                                       cGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATg             <  1:138676/138‑1 (MQ=255)
                                                                                                                              tAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAgg                           >  2:114238/1‑117 (MQ=255)
                                                                                                                              tAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAgg                           <  1:114238/117‑1 (MQ=255)
                                                                                                                                 aGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGa  <  2:528926/139‑1 (MQ=255)
                                                                                                                                     tGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCa                                 <  2:211442/104‑1 (MQ=255)
                                                                                                                                     tGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCa                                 >  1:211442/1‑104 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGA  >  NZ_CP009273/3747457‑3747724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑