Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,876,284	T→C	D269G (GAT→GGT)	dnaA ←	chromosomal replication initiator protein DnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,876,284	0	T	C	100.0%	91.7 / NA	26	D269G (GAT→GGT)	dnaA	chromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

GGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAA  >  NZ_CP009273/3876160‑3876409                                                                                                                             |                                                                                                                              ggCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATg                                                                                                                 <  2:458488/139‑1 (MQ=255)           atcaGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGa                                                                                                        <  2:374498/138‑1 (MQ=255)                     ccACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcag                                                                                             <  1:397037/139‑1 (MQ=255)                          cGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAggg                                                                                        >  2:445321/1‑139 (MQ=255)                          cGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAggg                                                                                        <  1:445321/139‑1 (MQ=255)                           gggTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                          <  2:487021/136‑1 (MQ=255)                           gggTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                          >  1:487021/1‑136 (MQ=255)                                tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTg                                                                                   >  2:427529/1‑138 (MQ=255)                                           cGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGaaa                                                                       <  2:410183/139‑1 (MQ=255)                                                                    cAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttat                                              <  2:486784/139‑1 (MQ=255)                                                                      aCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTttat                                              >  2:43898/1‑137 (MQ=255)                                                                         gAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                         <  1:43898/139‑1 (MQ=255)                                                                          aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                         >  2:455537/1‑138 (MQ=255)                                                                          aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                         >  1:350279/1‑138 (MQ=255)                                                                           aGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCaaa                                       <  1:427529/139‑1 (MQ=255)                                                                            gCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                               >  1:478482/1‑66 (MQ=255)                                                                            gCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                               <  2:478482/66‑1 (MQ=255)                                                                                    tCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGaa                               <  2:532916/138‑1 (MQ=255)                                                                                              cTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGAt                     <  1:149055/138‑1 (MQ=255)                                                                                                      cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg             >  1:432811/1‑138 (MQ=255)                                                                                                                tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTa       <  2:276017/134‑1 (MQ=255)                                                                                                                tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTAc      <  1:347242/135‑1 (MQ=255)                                                                                                                tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa  >  2:183272/1‑139 (MQ=255)                                                                                                                  tCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGaa  <  1:183272/137‑1 (MQ=255)                                                                                                                          cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACgg    >  2:321811/1‑127 (MQ=255)                                                                                                                          cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACgg    <  1:321811/127‑1 (MQ=255)                                                                                                                             |                                                                                                                              GGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAA  >  NZ_CP009273/3876160‑3876409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑