Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,937,289	A→G	noncoding (226/2904 nt)	BW25113_RS19530 →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,937,289	0	A	G	100.0%	48.7 / NA	24	noncoding (226/2904 nt)	BW25113_RS19530	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/9);  total (15/9)

TCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATG  >  NZ_CP009273/3937213‑3937413                                                                             |                                                                                                                             tCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt                                                                  <  2:50515/137‑1 (MQ=14)         tGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGt                                                        >  1:243690/1‑139 (MQ=17)         tGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGt                                                        >  2:97816/1‑139 (MQ=17)           aaTCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTg                                                       >  1:481801/1‑138 (MQ=17)                      tAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGGCTgg                                            >  1:161173/1‑138 (MQ=17)                       aaTGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGa                                           >  1:283308/1‑138 (MQ=17)                       aaTGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGa                                           >  2:513650/1‑138 (MQ=17)                       aaTGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGa                                           <  1:294644/138‑1 (MQ=17)                         tGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAg                                        <  1:513650/139‑1 (MQ=17)                         tGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAg                                        >  2:407065/1‑139 (MQ=17)                              cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGgcgcg                                   <  1:407065/139‑1 (MQ=17)                              cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGgcgcg                                   >  2:478657/1‑139 (MQ=17)                                    gggggAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATAc                             <  2:283308/139‑1 (MQ=17)                                     ggggAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATa                              >  1:271962/1‑137 (MQ=17)                                     ggggAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGGGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATa                              >  2:244961/1‑137 (MQ=17)                                      gggAACTGAAACATCTAAGTGCCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAg                           <  2:417533/139‑1 (MQ=17)                                           cTGAAACATCTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGa                      >  1:272030/1‑139 (MQ=17)                                                    cTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGgcgcg                                   >  2:335008/1‑117 (MQ=17)                                                    cTAAGTACCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGgcgcg                                   <  1:335008/117‑1 (MQ=17)                                                          aCCCCGAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTAcaca        >  2:520938/1‑138 (MQ=17)                                                               gAGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATg  <  2:243690/139‑1 (MQ=17)                                                                aGGAAAAGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATg  >  1:29505/1‑138 (MQ=17)                                                                      aGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGa                      >  1:525352/1‑112 (MQ=17)                                                                      aGAAATCGACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGa                      <  2:525352/112‑1 (MQ=17)                                                                             |                                                                                                                             TCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATG  >  NZ_CP009273/3937213‑3937413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑