Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,029,029	A→T	noncoding (139/1542 nt)	BW25113_RS19980 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,029,029	0	A	T	100.0%	28.8 / NA	15	noncoding (139/1542 nt)	BW25113_RS19980	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/9);  total (6/9)

CTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTA  >  NZ_CP009273/4028980‑4029152                                                  |                                                                                                                            cttTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCgg                                     <  2:28900/138‑1 (MQ=17)    tGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGa                                    <  2:124240/136‑1 (MQ=17)         aCGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGcc                              <  1:476487/137‑1 (MQ=17)           gAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGa                          <  2:410054/139‑1 (MQ=17)              tGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATgg                       >  1:541821/1‑139 (MQ=17)                            gTAATGTCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAgg         <  2:456197/139‑1 (MQ=17)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCg                                                     <  1:313892/89‑1 (MQ=18)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCg                                                     >  2:313892/1‑89 (MQ=18)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTgggg    >  2:347281/1‑138 (MQ=17)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTgggg    >  2:68090/1‑138 (MQ=17)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTgggg    >  2:71282/1‑138 (MQ=17)                                  tCTGGGAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt   <  2:257624/139‑1 (MQ=17)                                      ggAAACTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa  <  1:152578/136‑1 (MQ=17)                                           cTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGa                          <  1:324346/107‑1 (MQ=17)                                           cTGCCTGTTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGa                          >  2:324346/1‑107 (MQ=17)                                                  |                                                                                                                            CTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGTACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTA  >  NZ_CP009273/4028980‑4029152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑