Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,113,018	G→A	R84C (CGC→TGC)	ftsN ←	cell division protein FtsN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,113,018	0	G	A	100.0%	64.5 / NA	17	R84C (CGC→TGC)	ftsN	cell division protein FtsN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/9);  total (8/9)

GAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAG  >  NZ_CP009273/4112904‑4113130                                                                                                                   |                                                                                                                 gaagCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTtggtgg                                                                                          <  2:550178/139‑1 (MQ=255)                   gtgtCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCAc                                                                        >  1:329879/1‑139 (MQ=255)                        aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCt                                                                   >  1:85046/1‑139 (MQ=255)                             cTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCtt                                                              >  1:534972/1‑139 (MQ=255)                                           cttcacCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa                                                <  2:534972/139‑1 (MQ=255)                                                          aaGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgtg                                 <  1:6470/139‑1 (MQ=255)                                                                cTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                            <  2:329879/138‑1 (MQ=255)                                                                     ggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                       <  2:483830/138‑1 (MQ=255)                                                                       cgcacgcaCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAg                    >  2:564994/1‑139 (MQ=255)                                                                        gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAg                    >  1:297577/1‑138 (MQ=255)                                                                           cgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtcttct                                         <  1:297967/114‑1 (MQ=255)                                                                           cgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtcttct                                         >  2:297967/1‑114 (MQ=255)                                                                               cTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc            <  2:297577/139‑1 (MQ=255)                                                                                tCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacca           >  2:504676/1‑139 (MQ=255)                                                                                         gCGTCTTTCCAGCTCTTTAATGTAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAg  <  2:85046/139‑1 (MQ=255)                                                                                                               tAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgtg                                 <  2:455278/86‑1 (MQ=255)                                                                                                               tAGCACCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTtgtg                                 >  1:455278/1‑86 (MQ=255)                                                                                                                   |                                                                                                                 GAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAG  >  NZ_CP009273/4112904‑4113130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑