Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	303,602	T→G	N481T (AAC→ACC)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	303,602	0	T	G	100.0%	53.2 / NA	17	N481T (AAC→ACC)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/7);  total (10/7)

CTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGC  >  NZ_CP009273/303470‑303729                                                                                                                                       |                                                                                                                                cTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCgg                                                                                                                           <  1:335942/139‑1 (MQ=255)            ttGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGGTGCTGGAAGTGCCCAGCTTCGGC‑‑CCCAGTGAGGTTAGGGTGGGGGTGGCGCCGATTGCACCGGGGTCGGCCACGCtcagg                                                                                                                >  2:335942/1‑135 (MQ=255)            ttGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCCGCTTCGAC‑‑CACAGTGAGTTCAGGGTGAGTGGGCCGCCGGTTGCACGGGGGTCGGCATCGCTACGt                                                                                                                >  1:451698/1‑139 (MQ=255)                               taataaTGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCCTCGAC‑‑CACAATGGGTTTCGGTTGAGTGTGGCGCCGATTGCACGGGTGTCGGGCTCGCTACCTCGCAATTGATTGCCCATGc                                                                                             >  1:393901/1‑139 (MQ=255)                                    atGGCTGTTGTAACGGCGGTTTTCCTTGTTGCTGGTGCTGAATTTGGCCAGCTTTTGC‑‑CACAGTGGGTTTCGGTTGGGTGTGCCGCCTGTTTCACCGGGGGCGGCATCGCCCCGTTGTAATTTTTTTGCAATACggggt                                                                                        >  2:479091/1‑137 (MQ=255)                                        cagttgtAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTGGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGtttttt                                                                                    <  2:533570/137‑1 (MQ=255)                                           ttgtAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCt                                                                                 <  1:479091/139‑1 (MQ=255)                                                        catcatTGTAGCTGATGCTGAATGTGCCCCGCTTCGAC‑‑CACAGGGAGTTCAGGTTGAGTGTGCCGCCGCTTGCACGGGGGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGGTTTTTTCTGATTAACCCCCAg                                                                    >  1:26000/1‑139 (MQ=255)                                                         atcatTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCcaca                                                                     <  1:291802/139‑1 (MQ=255)                                                                 aGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTa                                                             >  1:543206/1‑137 (MQ=255)                                                                      aTGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTc                                                      >  1:541944/1‑139 (MQ=255)                                                                                                        gTTCAGGTTGAGTGTGCCGACGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGAtgctg                      <  1:228909/139‑1 (MQ=255)                                                                                                         ttCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                    <  1:440845/92‑1 (MQ=255)                                                                                                         ttCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                    >  2:440845/1‑92 (MQ=255)                                                                                                             ggTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCa                 >  1:280980/1‑139 (MQ=255)                                                                                                                        ccgccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCa       <  1:442475/138‑1 (MQ=255)                                                                                                                              aTTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGc  >  1:304021/1‑137 (MQ=255)                                                                                                                                       |                                                                                                                                CTGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGAC‑‑CACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCACTGGC  >  NZ_CP009273/303470‑303729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑