Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,389,340	A→G	E91G (GAA→GGA)	miaA →	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,389,340	0	A	G	100.0%	101.5 / NA	28	E91G (GAA→GGA)	miaA	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

CAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCC  >  NZ_CP009273/4389209‑4389448                                                                                                                                     |                                                                                                             cAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGc                                                                                                        <  2:131645/138‑1 (MQ=255)    aGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGa                                                                                                     >  1:534271/1‑138 (MQ=255)           gatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACcg                                                                                             >  2:514602/1‑139 (MQ=255)              atCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCCCCgcgg                                                                                          >  2:252425/1‑139 (MQ=255)                cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGGAATGGCCGATATCACCgcggc                                                                                         >  2:458409/1‑139 (MQ=255)                cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCgcggcg                                                                                        >  1:141609/1‑139 (MQ=255)                cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCgcggcg                                                                                        >  1:181157/1‑139 (MQ=255)                cGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCgcggcg                                                                                        >  1:249212/1‑139 (MQ=255)                    aCGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCggcggggc                                                                                    <  2:156557/139‑1 (MQ=255)                     cGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCggcggggcg                                                                                   >  2:372206/1‑139 (MQ=255)                        cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATGCGGCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGAt                                                                                <  2:434658/139‑1 (MQ=255)                          aaGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATcc                                                                              <  2:534271/139‑1 (MQ=255)                          aaGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATcc                                                                              <  2:467002/139‑1 (MQ=255)                          aaGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATcc                                                                              <  1:178898/139‑1 (MQ=255)                                       aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                 <  1:514602/139‑1 (MQ=255)                                       aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                 <  2:249212/139‑1 (MQ=255)                                       aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                 <  1:14953/139‑1 (MQ=255)                                        agagTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTggg                                                                 <  1:458409/139‑1 (MQ=255)                                         gagTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCg                                                               <  1:372206/139‑1 (MQ=255)                                               cTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACg                                                          <  1:158786/138‑1 (MQ=255)                                                                       gATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCGGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTgg                                 <  2:14044/139‑1 (MQ=255)                                                                          atTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGaa                               <  2:345837/138‑1 (MQ=255)                                                                          atTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGaa                               >  1:345837/1‑138 (MQ=255)                                                                             cgcgATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAggg                            <  2:141609/138‑1 (MQ=255)                                                                                       cGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGGACGATGTTGTATTTCAAGGCATTGCTGGAAGGGGTGTcgccgc                  >  2:399399/1‑138 (MQ=255)                                                                                                      ctgctgATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTg                                                                   <  2:134638/74‑1 (MQ=255)                                                                                                      ctgctgATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTg                                                                   >  1:134638/1‑74 (MQ=255)                                                                                                      ctgctgATTTTCGCCGCGATGCGCTGGC‑GGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGAccc  >  1:155504/1‑139 (MQ=255)                                                                                                                                     |                                                                                                             CAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGC‑GGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCC  >  NZ_CP009273/4389209‑4389448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑