Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,401,268	T→G	G50G (GGT→GGG)	yjfK →	YjfK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,401,268	0	T	G	100.0%	77.0 / NA	22	G50G (GGT→GGG)	yjfK	YjfK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/11);  total (11/11)

CAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACA  >  NZ_CP009273/4401162‑4401395                                                                                                           |                                                                                                                                cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACAt                                                                                                 >  1:160477/1‑139 (MQ=255) cAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACAt                                                                                                 >  2:153026/1‑139 (MQ=255)  aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATc                                                                                                >  1:557262/1‑139 (MQ=255)                              cATCTCAATAGTGGCTTTACGCTCGACCCGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTc                                                                     <  2:299127/138‑1 (MQ=255)                               aTCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCg                                                                   <  1:153026/139‑1 (MQ=255)                                           gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                                <  1:342377/82‑1 (MQ=255)                                           gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                                >  2:342377/1‑82 (MQ=255)                                           gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt                                                        >  2:459494/1‑138 (MQ=255)                                           gCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACtt                                                        >  2:403985/1‑138 (MQ=255)                                              ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTg                                                                                          >  2:20069/1‑101 (MQ=255)                                              ttACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTg                                                                                          <  1:20069/101‑1 (MQ=255)                                                aCGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAg                                                                                                       <  1:387207/86‑1 (MQ=255)                                                aCGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAg                                                                                                       >  2:387207/1‑86 (MQ=255)                                                 cGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCg                                                  <  1:50919/138‑1 (MQ=255)                                                                cgtttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAAt                                  >  2:508464/1‑139 (MQ=255)                                                                  tttcgtttGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATc                                 >  2:459670/1‑138 (MQ=255)                                                                                 gATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGgcggcg                 <  2:530898/139‑1 (MQ=255)                                                                                  aTGAATTGCTGATTTGGCTGGCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGa                <  1:453905/139‑1 (MQ=255)                                                                                             aTTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtga       <  2:557262/137‑1 (MQ=255)                                                                                                 cgcTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACa  <  1:459670/138‑1 (MQ=255)                                                                                                      ccGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAAt                              >  2:299762/1‑105 (MQ=255)                                                                                                      ccGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAAt                              <  1:299762/105‑1 (MQ=255)                                                                                                           |                                                                                                                                CAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGACA  >  NZ_CP009273/4401162‑4401395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑