Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,498,429	T→G	R2S (AGA→AGC)	BW25113_RS22240 ←	IS30‑like element IS30 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,498,429	0	T	G	100.0%	35.6 / NA	11	R2S (AGA→AGC)	BW25113_RS22240	IS30‑like element IS30 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/9);  total (2/9)

TTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAG  >  NZ_CP009273/4498374‑4498558                                                        |                                                                                                                                  ttCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAat                                                 <  2:157921/138‑1 (MQ=21)    cGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAatatat                                             <  1:510979/139‑1 (MQ=37)                gTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGc                                 <  2:145777/139‑1 (MQ=255)                gTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGc                                 >  2:407743/1‑139 (MQ=255)                    aaaaaCAGAGGCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAg                              <  1:438545/138‑1 (MQ=255)                             ggCTTTTTCCTCTGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTaa                    <  2:400336/139‑1 (MQ=255)                                      ctctGCTGTAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACg           <  2:524493/139‑1 (MQ=255)                                              tAAATGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCa   <  1:407743/139‑1 (MQ=255)                                                aaTGTTCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTGTCGCATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTGTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAg  <  1:263798/138‑1 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCttttt                                                      >  1:564281/1‑81 (MQ=255)                                                     tCGGCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCttttt                                                      <  2:564281/81‑1 (MQ=255)                                                        |                                                                                                                                  TTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAG  >  NZ_CP009273/4498374‑4498558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑