Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 393,907 A→G K194E (AAA→GAA)  yaiW → surface‑exposed outer membrane lipoprotein YaiW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273393,9070AG100.0% 98.0 / NA 27K194E (AAA→GAA) yaiWsurface‑exposed outer membrane lipoprotein YaiW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

CGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCT  >  NZ_CP009273/393771‑394039
                                                                                                                                        |                                                                                                                                    
cgcgATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGgaa                                                                                                                                    <  2:76746/139‑1 (MQ=255)
  cgATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAAt                                                                                                                                  >  1:474226/1‑139 (MQ=255)
                         aTGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAg                                                                                                           >  2:520225/1‑139 (MQ=255)
                                  aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGgaa                                                                                                                                    <  1:284258/105‑1 (MQ=255)
                                  aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGgaa                                                                                                                                    >  2:284258/1‑105 (MQ=255)
                                  aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTc                                                                                                  >  1:230111/1‑139 (MQ=255)
                                  aTGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTc                                                                                                  >  2:377258/1‑139 (MQ=255)
                                        cAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCcgcg                                                                                            <  2:91519/139‑1 (MQ=255)
                                           aCGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCgcggcg                                                                                         >  2:169002/1‑139 (MQ=255)
                                                     gTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGtt                                                                               <  1:520225/139‑1 (MQ=255)
                                                     gTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGtt                                                                               <  1:377258/139‑1 (MQ=255)
                                                             aaTCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACtt                                                                       <  2:230111/139‑1 (MQ=255)
                                                                     gcgcACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTa                                                               >  2:246240/1‑139 (MQ=255)
                                                                                     aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                                 >  1:177738/1‑137 (MQ=255)
                                                                                     aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                                 >  1:406418/1‑137 (MQ=255)
                                                                                      tGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAg                                              <  2:177738/139‑1 (MQ=255)
                                                                                              aGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                      <  2:106380/139‑1 (MQ=255)
                                                                                                cATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGtt                                                                               <  1:175111/96‑1 (MQ=255)
                                                                                                cATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGtt                                                                               >  2:175111/1‑96 (MQ=255)
                                                                                                 aTTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCAc                                   <  1:236316/139‑1 (MQ=255)
                                                                                                   tGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCAcc                                  >  2:356470/1‑138 (MQ=255)
                                                                                                               accgCATACCAAAGGGAATCCGTGGGAAATGGACGGTACAGTCAGTCAGGCAGTCTTCAGACGCCGCGGCGGGTTGGGGGTTGGGACTTAACATTTACTGAATTATCCCGCCAGTTATAGCGCACCGGTATACCAtttt                     >  2:239752/4‑139 (MQ=255)
                                                                                                                             ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                      >  1:387458/1‑108 (MQ=255)
                                                                                                                             ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                      <  2:387458/108‑1 (MQ=255)
                                                                                                                             ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                      >  2:419437/1‑108 (MQ=255)
                                                                                                                             ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                      <  1:419437/108‑1 (MQ=255)
                                                                                                                                 tCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGAttt             <  1:556982/129‑1 (MQ=255)
                                                                                                                                 tCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGAttt             >  2:556982/1‑129 (MQ=255)
                                                                                                                                  ccGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgctggct  >  2:130386/1‑139 (MQ=255)
                                                                                                                                  ccGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgctggc   <  2:111812/138‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCT  >  NZ_CP009273/393771‑394039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑