Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 415,362 T→C S106P (TCC→CCC)  brnQ → branched‑chain amino acid transporter carrier protein BrnQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273415,3620TC100.0% 102.9 / NA 29S106P (TCC→CCC) brnQbranched‑chain amino acid transporter carrier protein BrnQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/19);  total (10/19)

AGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGCTGCTGGATACCGTGGGCAA  >  NZ_CP009273/415226‑415492
                                                                                                                                        |                                                                                                                                  
aGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTccc                                                                                                                                  >  2:430956/1‑139 (MQ=255)
    gcgcTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCttt                                                                                                                               >  1:314379/1‑138 (MQ=255)
                   ggcggcGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTgcg                                                                                                                <  2:314379/138‑1 (MQ=255)
                   ggcggcGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTgcg                                                                                                                <  1:515564/138‑1 (MQ=255)
                                    ccTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTcc                                                                                              >  2:170543/1‑139 (MQ=255)
                                     cTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCcg                                                                                             >  1:290227/1‑139 (MQ=255)
                                              ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                                                                                    >  1:203760/1‑139 (MQ=255)
                                              ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                                                                                    <  2:414691/139‑1 (MQ=255)
                                              ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                                                                                    <  2:203760/139‑1 (MQ=255)
                                              ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                                                                                    <  2:163935/139‑1 (MQ=255)
                                                                         ctgGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCg                                                         >  2:153370/1‑139 (MQ=255)
                                                                           ggCAACGGTTTGTTACCTAGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGAGTGCGCCGCTGACGGCGGATTCCGCGCTGCCGCTGTTTAGCTACAGCCTGGTCTATTTCGc                                                        >  2:527165/1‑138 (MQ=255)
                                                                                      gTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTc                                             <  1:170543/138‑1 (MQ=255)
                                                                                          ccTGGAGGCGGGGCCTCTTTTCGCTACGACGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTTTATTTCGCTATCGTTATTCTGGt                                         <  1:527165/138‑1 (MQ=255)
                                                                                             ggCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTg                                                                                  <  1:563328/94‑1 (MQ=255)
                                                                                             ggCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTg                                                                                  >  2:563328/1‑94 (MQ=255)
                                                                                               cGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGct                                   <  2:311248/139‑1 (MQ=255)
                                                                                               cGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGct                                   <  2:456996/139‑1 (MQ=255)
                                                                                               cGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGct                                   <  2:128457/139‑1 (MQ=255)
                                                                                                    gggCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTAt                               <  1:416786/138‑1 (MQ=255)
                                                                                                     ggCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCt                                                                           <  1:497083/93‑1 (MQ=255)
                                                                                                     ggCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCt                                                                           >  2:497083/1‑93 (MQ=255)
                                                                                                          cTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCa                        <  1:361979/139‑1 (MQ=255)
                                                                                                             ttCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAg                      <  2:35171/138‑1 (MQ=255)
                                                                                                               cGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAgct                    <  1:153370/138‑1 (MQ=255)
                                                                                                                         cgTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTAt                                                     >  1:528331/1‑95 (MQ=255)
                                                                                                                         cgTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTAt                                                     <  2:528331/95‑1 (MQ=255)
                                                                                                                                cTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGCTGCTGGATACCGTGGGCaa  <  2:359105/139‑1 (MQ=255)
                                                                                                                                   ccGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGtt                                        <  2:327726/98‑1 (MQ=255)
                                                                                                                                   ccGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGtt                                        >  1:327726/1‑98 (MQ=255)
                                                                                                                                        |                                                                                                                                  
AGTGGCGCTGGCAAAAGTTGGCGGCGGTGTTGACAGCCTCAGCACGCCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGCTATCGTTATTCTGGTTTCGCTCTATCCGGGCAAGCTGCTGGATACCGTGGGCAA  >  NZ_CP009273/415226‑415492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑