| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 446,387 | T→C | D227G (GAT→GGT) | cyoA ← | cytochrome o ubiquinol oxidase subunit II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 446,387 | 0 | T | C | 94.7% | 55.9 / ‑5.6 | 19 | D227G (GAT→GGT) | cyoA | cytochrome o ubiquinol oxidase subunit II |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (8/10); minor base G (0/1); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAAT > NZ_CP009273/446251‑446520 | gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGAccc < 1:3237/139‑1 (MQ=255)gTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGAccc < 1:49884/139‑1 (MQ=255) gTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCgg < 1:450023/136‑1 (MQ=255) aCGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa < 1:522332/139‑1 (MQ=255) gaAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTg < 1:454313/139‑1 (MQ=255) gcgcGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCt < 1:71504/139‑1 (MQ=255) ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa > 1:104372/1‑65 (MQ=255) ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa < 2:104372/65‑1 (MQ=255) ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCg < 2:521748/95‑1 (MQ=255) ggTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCg > 1:521748/1‑95 (MQ=255) gtgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGt > 2:66648/1‑139 (MQ=255) tgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa < 2:235361/63‑1 (MQ=255) tgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCaa > 1:235361/1‑63 (MQ=255) tgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGtt > 2:160718/1‑139 (MQ=255) tgtTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGtt > 2:176071/1‑139 (MQ=255) ttCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTgg < 1:160718/139‑1 (MQ=255) gCTTTTGCGACCCACTGGTCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAgg < 1:66648/138‑1 (MQ=255) ttcGACGCACTGGTCAAATGCGGCGCGAGCCGGTGTTGCAATAGCTTTGAACATCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATGCCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGt < 2:410808/136‑1 (MQ=255) tCGAATGCGGCGCGACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCa < 1:343881/139‑1 (MQ=255) cgcgACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAAt > 1:469821/1‑139 (MQ=255) cgcgACCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAAt > 1:79288/1‑139 (MQ=255) | GTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGATACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAAT > NZ_CP009273/446251‑446520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |