Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	478,146	A→C	F572V (TTT→GTT)	acrB ←	efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	478,146	0	A	C	100.0%	144.0 / NA	39	F572V (TTT→GTT)	acrB	efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/23);  total (16/23)

GCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCG  >  NZ_CP009273/478012‑478276                                                                                                                                       |                                                                                                                                   gccgaagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACg                                                                                                                                >  1:136168/1‑139 (MQ=255)   cgaagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGcc                                                                                                                              <  2:490847/139‑1 (MQ=255)    gaagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGccc                                                                                                                             <  2:193792/139‑1 (MQ=255)              aCGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCAt                                                                                                                   <  1:464395/139‑1 (MQ=255)                  cGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTgg                                                                                                               >  2:231262/1‑139 (MQ=255)                               aaCGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCt                                                                                                   <  1:477602/138‑1 (MQ=255)                                   ttgttCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCa                                                                                              <  2:555192/139‑1 (MQ=255)                                            tctttGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACg                                                                                      <  2:158741/138‑1 (MQ=255)                                             ctttGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGa                                                                                     <  2:329712/138‑1 (MQ=255)                                                tGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGaa                                                                                    <  2:418190/136‑1 (MQ=255)                                                                 gTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCa                                                                >  1:431174/1‑139 (MQ=255)                                                                   tACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCAc                                                               >  2:436074/1‑138 (MQ=255)                                                                   tACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCAc                                                               >  1:344379/1‑138 (MQ=255)                                                                     ccTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACgat                                                            >  1:373219/1‑139 (MQ=255)                                                                        cATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAg                                                                                 >  2:139662/1‑115 (MQ=255)                                                                        cATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAg                                                                                 <  1:139662/115‑1 (MQ=255)                                                                         aTTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        <  1:231262/139‑1 (MQ=255)                                                                         aTTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                        <  2:431174/139‑1 (MQ=255)                                                                             aGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGa                                                     <  2:211516/138‑1 (MQ=255)                                                                              gCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATa                                                   <  1:436303/139‑1 (MQ=255)                                                                                  tttCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAg                                                >  2:88244/1‑138 (MQ=255)                                                                                    tCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCAc                                             >  1:330693/1‑139 (MQ=255)                                                                                       gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCa                                                                                              <  1:163915/87‑1 (MQ=255)                                                                                       gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCa                                                                                              >  2:163915/1‑87 (MQ=255)                                                                                        tgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCAcc                                            <  2:373219/136‑1 (MQ=255)                                                                                                  tGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTc                                                                                                                        <  2:29259/50‑1 (MQ=255)                                                                                                  tGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTc                                                                                                                        >  1:29259/1‑50 (MQ=255)                                                                                                  tGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACg                                                                                      <  1:98087/84‑1 (MQ=255)                                                                                                  tGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACg                                                                                      >  2:98087/1‑84 (MQ=255)                                                                                                  tGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCg                               >  1:389102/1‑139 (MQ=255)                                                                                                         cACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTgcg                        <  2:389102/139‑1 (MQ=255)                                                                                                         cACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGCAACGCCCCGTACTgcg                        >  1:390746/1‑139 (MQ=255)                                                                                                              gctgGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATa                                                   <  1:99461/107‑1 (MQ=255)                                                                                                              gctgGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATa                                                   >  2:99461/1‑107 (MQ=255)                                                                                                              gctgGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGaa                   >  1:522186/1‑139 (MQ=255)                                                                                                                           ccATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACTCCCCGTACT‑‑GCAGAATACCGCCTACGCTGt    <  1:104509/139‑1 (MQ=255)                                                                                                                             aTGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGt    <  2:98311/139‑1 (MQ=255)                                                                                                                                gTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCg  <  2:427881/138‑1 (MQ=255)                                                                                                                                gTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCg  <  2:136168/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GCCGAAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCTGTCG  >  NZ_CP009273/478012‑478276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑