Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	609,496	A→C	L33F (TTA→TTC)	ybdZ →	enterobactin biosynthesis protein YbdZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	609,496	0	A	C	100.0%	91.2 / NA	25	L33F (TTA→TTC)	ybdZ	enterobactin biosynthesis protein YbdZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/13);  total (12/13)

CCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAT  >  NZ_CP009273/609362‑609614                                                                                                                                       |                                                                                                                       ccTCTGGCAACCCCTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg                                                                                                                    <  2:262890/139‑1 (MQ=255)       gCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCt                                                                                                              <  2:56029/139‑1 (MQ=255)                                       ggCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCg                                                                              >  2:129873/1‑139 (MQ=255)                                       ggCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCg                                                                              >  2:505973/1‑139 (MQ=255)                                        gCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGt                                                                             <  2:479331/139‑1 (MQ=255)                                            tCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTg                                                                         >  1:327112/1‑139 (MQ=255)                                            tCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTg                                                                         >  1:401911/1‑139 (MQ=255)                                                  aTCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcagca                                                                   <  1:129873/139‑1 (MQ=255)                                                        tCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCt                                                             >  2:369755/1‑139 (MQ=255)                                                              atCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg                                                                                                                    >  2:131896/1‑78 (MQ=255)                                                              atCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCgg                                                                                                                    <  1:131896/78‑1 (MQ=255)                                                               tCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGcc                                                      <  2:401911/139‑1 (MQ=255)                                                                ccGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAg                                                                                       <  1:520715/105‑1 (MQ=255)                                                                ccGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAg                                                                                       >  2:520715/1‑105 (MQ=255)                                                                   cAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACt                                                  >  2:124467/1‑139 (MQ=255)                                                                       gAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCg                                              <  1:415061/139‑1 (MQ=255)                                                                        aGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcag                                                                     <  1:234931/115‑1 (MQ=255)                                                                        aGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCcag                                                                     >  2:234931/1‑115 (MQ=255)                                                                                       gcgcAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACcgac                               >  2:282233/1‑138 (MQ=255)                                                                                                gcAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc                     <  1:124467/139‑1 (MQ=255)                                                                                                             aGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCaggagg        <  2:450642/139‑1 (MQ=255)                                                                                                             aGTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCaggagg        <  1:369755/139‑1 (MQ=255)                                                                                                              gTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc                     <  2:386317/125‑1 (MQ=255)                                                                                                              gTCTGTGGCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTAcc                     >  1:386317/1‑125 (MQ=255)                                                                                                                    ggCCGCAACAATGCGTCTTCCCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAt  >  1:166920/1‑138 (MQ=255)                                                                                                                                       |                                                                                                                       CCTCTGGCAACCACTTTTCCATGACAGGAGTTGAATATGGCATTCAGTAATCCCTTCGATGATCCGCAGGGAGCGTTTTACATATTGCGCAATGCGCAGGGGCAATTCAGTCTGTGGCCGCAACAATGCGTCTTACCGGCAGGCTGGGACATTGTGTGTCAGCCGCAGTCACAGGCGTCCTGCCAGCAGTGGCTGGAAGCCCACTGGCGTACTCTGACACCGACGAATTTTACCCAGTTGCAGGAGGCACAAT  >  NZ_CP009273/609362‑609614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑