Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 625,777 A→G D143G (GAC→GGC)  cstA → pyruvate/proton symporter CstA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273625,7770AG100.0% 75.3 / NA 22D143G (GAC→GGC) cstApyruvate/proton symporter CstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/10);  total (12/10)

GGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGCGATGATCGTGGTGAAAGCCCT  >  NZ_CP009273/625641‑625906
                                                                                                                                        |                                                                                                                                 
ggAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCg                                                                                                                                 <  1:414688/139‑1 (MQ=255)
       gTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCGGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGtcgctc                                                                                                                          >  2:309576/1‑139 (MQ=255)
       gTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGtcgctc                                                                                                                          <  1:65005/139‑1 (MQ=255)
        tCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGtcgctcg                                                                                                                         >  1:385390/1‑139 (MQ=255)
                         tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAg                                                                                                                >  1:175507/1‑131 (MQ=255)
                         tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAg                                                                                                                <  2:175507/131‑1 (MQ=255)
                                                         atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAg                                                                                                                >  2:220096/1‑99 (MQ=255)
                                                         atgatCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAg                                                                                                                <  1:220096/99‑1 (MQ=255)
                                                                      tCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTg                                                           >  1:245339/1‑139 (MQ=255)
                                                                                                      gATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGc                           >  2:11566/1‑139 (MQ=255)
                                                                                                           cATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGtcgct                                                                                                                           <  1:152009/38‑1 (MQ=255)
                                                                                                           cATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGtcgct                                                                                                                           >  2:152009/1‑38 (MQ=255)
                                                                                                           cATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGcc                                                                                         <  2:8864/72‑1 (MQ=255)
                                                                                                           cATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGcc                                                                                         >  1:8864/1‑72 (MQ=255)
                                                                                                           cATGGTGCTGTTTGTTTCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGc                       >  1:149136/1‑138 (MQ=255)
                                                                                                                         tttCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGCGATGATCGTGGTGa         <  1:193247/138‑1 (MQ=255)
                                                                                                                          ttCTACGCGCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGCGATGATCGTGGTGa         >  1:568357/1‑137 (MQ=255)
                                                                                                                               cgcgCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGAt                                                  <  2:130479/91‑1 (MQ=255)
                                                                                                                               cgcgCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGAt                                                  >  1:130479/1‑91 (MQ=255)
                                                                                                                               cgcgCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAt                                         >  1:210992/1‑100 (MQ=255)
                                                                                                                               cgcgCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCAt                                         <  2:210992/100‑1 (MQ=255)
                                                                                                                               cgcgCCGTGGCGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGCGATGATCGTGGTGAAAGCCCt  <  2:245339/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCGGTGCAGGATTTCATGGTGCTGTTTGTTTCTACGCGCCGTGACGGTCGCTCGCTGGGTGAGCTGGTCAAAGAAGAGATGGGGCCAACCGCCGGGGTGATTGCGCTGGTGGCCTGCTTTATGATCATGGTCATTATCCTTGCAGTGCTGGCGATGATCGTGGTGAAAGCCCT  >  NZ_CP009273/625641‑625906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑