Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	37,524	T→G	S101R (AGC→CGC)	caiC ←	crotonobetaine/carnitine‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	37,524	0	T	G	100.0%	120.4 / NA	34	S101R (AGC→CGC)	caiC	crotonobetaine/carnitine‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/18);  total (16/18)

ATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37402‑37655                                                                                                                           |                                                                                                                                    aTCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCGCTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAgg                                                                                                                     <  2:196469/139‑1 (MQ=255) aTCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAgg                                                                                                                     <  2:10265/139‑1 (MQ=255) aTCTGTCAGGCAAATATGCAGCAATTGAATGGCATCTTCCTGCTGAATCTGGTGATACATAGGATAGAATTGCGCACTGGTCGCCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAgg                                                                                                                     <  2:87006/139‑1 (MQ=255)                   ccGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCAc                                                                                                    >  2:172310/1‑138 (MQ=255)                    cGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCa                                                                                                  >  2:97609/1‑139 (MQ=255)                              tGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAgg                                                                                                                     <  1:468438/110‑1 (MQ=255)                              tGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAgg                                                                                                                     >  2:468438/1‑110 (MQ=255)                              tGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCgcgc                                                                                         >  1:481101/1‑138 (MQ=255)                                      tCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTg                                                                                >  2:431380/1‑139 (MQ=255)                                        ctgctgAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGc                                                                               <  2:169442/138‑1 (MQ=255)                                           ctgAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         <  2:85953/109‑1 (MQ=255)                                           ctgAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATc                                                                                                         >  1:85953/1‑109 (MQ=255)                                              aaTCTGTTGATACATCGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGccc                                                                         >  1:243051/1‑138 (MQ=255)                                              aaTCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGccc                                                                         >  1:429897/1‑138 (MQ=255)                                                         aCATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCaa                                                               >  2:227414/1‑137 (MQ=255)                                                              ggATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATa                                                         >  1:130116/1‑138 (MQ=255)                                                                 tAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAAtt                                                     <  1:42214/139‑1 (MQ=255)                                                                    aaTTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCg                                                  >  2:545989/1‑139 (MQ=255)                                                                      ttGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCggg                                                <  1:545989/139‑1 (MQ=255)                                                                         cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  2:99434/1‑139 (MQ=255)                                                                         cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAg                                             >  1:57769/1‑139 (MQ=255)                                                                         cgcACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATCTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCa                                              <  1:322112/138‑1 (MQ=255)                                                                                gTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCg                                       <  2:481101/138‑1 (MQ=255)                                                                                   aCCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCgaga                                    <  2:243051/138‑1 (MQ=255)                                                                                    ccAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATg                                  <  2:303811/139‑1 (MQ=255)                                                                                    ccAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATg                                  <  1:520562/139‑1 (MQ=255)                                                                                          ggCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCaa                                                               >  2:173450/1‑104 (MQ=255)                                                                                          ggCACGCCTGGCTATTTTGCAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCaa                                                               <  1:173450/104‑1 (MQ=255)                                                                                                             cAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCg          >  1:251054/1‑138 (MQ=255)                                                                                                             cAGGATCCACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGa         <  1:99434/139‑1 (MQ=255)                                                                                                                    cACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCa  <  2:130116/139‑1 (MQ=255)                                                                                                                    cACGCGCGTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCa  <  2:429897/139‑1 (MQ=255)                                                                                                                       gcgcGTTCCTCGCACAACAGGCGGGCGTTAATCgg                                                                                                       >  2:22280/1‑35 (MQ=255)                                                                                                                       gcgcGTTCCTCGCACAACAGGCGGGCGTTAATCgg                                                                                                       <  1:22280/35‑1 (MQ=255)                                                                                                                           |                                                                                                                                    ATCTGTCAGGCAAATGTGCCGCAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCCTGGCTATTTTGCAGGATCCACGCGCTTTCCTCGCACAACAGGCGGGCGTTAATCGGCACCATAATCGCGCCAATTTTTGCCAGCCCGAACCAGCAAAAGATAAATTCCGGGCAGTTGTCGAGATGTAGTGCAACCTTGTCGCCTTTGCGAATCCCCA  >  NZ_CP009273/37402‑37655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑