Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	724,034	A→C	V52G (GTC→GGC)	kdpA ←	potassium‑transporting ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	724,034	0	A	C	100.0%	28.4 / NA	9	V52G (GTC→GGC)	kdpA	potassium‑transporting ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/4);  total (5/4)

GCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAA  >  NZ_CP009273/723945‑724152                                                                                          |                                                                                                                       gCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCggg                                                                          >  2:21462/1‑136 (MQ=255)                gCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAAt                                                        >  1:46564/1‑139 (MQ=255)                      ccAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa                                                                  >  1:392437/1‑123 (MQ=255)                      ccAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa                                                                  <  2:392437/123‑1 (MQ=255)                                                             atTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCa                     >  1:255508/1‑129 (MQ=255)                                                             atTGCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCa                     <  2:255508/129‑1 (MQ=255)                                                                gCTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAaca         <  2:212868/138‑1 (MQ=255)                                                                 cTTCCAGTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAacac        <  2:492408/138‑1 (MQ=255)                                                                       gTTCATCTCACGGTCAGAGCCGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACAAGTaa  >  1:242725/1‑138 (MQ=255)                                                                                          |                                                                                                                       GCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAA  >  NZ_CP009273/723945‑724152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑