| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,041,277 | T→C | intergenic (‑18/+28) | gfcE ← / ← gfcD | polysaccharide export protein/YjbH domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,041,277 | 0 | T | C | 84.6% | 26.3 / 0.4 | 13 | intergenic (‑18/+28) | gfcE/gfcD | polysaccharide export protein/YjbH domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (7/4); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01 | |||||||||||
TCCTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCG > NZ_CP009273/1041156‑1041411 | tCCTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCAATGTAATGTTATGaa > 2:436913/1‑139 (MQ=255) ccTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCAATGTAATGTTATGAAt > 2:467329/1‑139 (MQ=255) aCTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCAATGTAATGTTATGAATTACGTc < 1:467329/138‑1 (MQ=255) gTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCaatgtaat > 1:119312/1‑119 (MQ=255) gTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCaatgtaat < 2:119312/119‑1 (MQ=255) tCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTg > 2:289418/1‑138 (MQ=255) gTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGc < 1:208223/126‑1 (MQ=255) gTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGc > 2:208223/1‑126 (MQ=255) aaTGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCa < 1:289418/139‑1 (MQ=255) aTCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCAt > 1:483881/1‑110 (MQ=255) aTCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCAt < 2:483881/110‑1 (MQ=255) aTCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAaccacc > 1:68722/1‑139 (MQ=255) tCAGCCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACcg > 1:302851/1‑139 (MQ=255) | TCCTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCG > NZ_CP009273/1041156‑1041411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |