Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,060,660	T→C	L390P (CTC→CCC)	yccE →	YccE family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,060,660	0	T	C	100.0%	70.4 / NA	20	L390P (CTC→CCC)	yccE	YccE family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

TAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTT  >  NZ_CP009273/1060529‑1060795                                                                                                                                    |                                                                                                                                        tAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCcctcct                                                                                                                                  <  2:521332/139‑1 (MQ=255)              ttatttatTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                     <  2:316897/139‑1 (MQ=255)                            tAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTa                                                                                                       <  1:427015/139‑1 (MQ=255)                                  tgtgGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCt                                                                                                                      <  2:319733/118‑1 (MQ=255)                                  tgtgGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCt                                                                                                                      >  1:319733/1‑118 (MQ=255)                                  tgtgGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTa                                                                                                       <  1:240783/133‑1 (MQ=255)                                  tgtgGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTa                                                                                                       >  2:240783/1‑133 (MQ=255)                                     ggCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCtt                                                                                                               >  1:559540/1‑122 (MQ=255)                                     ggCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCtt                                                                                                               <  2:559540/122‑1 (MQ=255)                                                          tgaatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCtt                                                                                                               >  1:413484/1‑101 (MQ=255)                                                          tgaatCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCtt                                                                                                               <  2:413484/101‑1 (MQ=255)                                                                                        gTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCa                                            >  2:502353/1‑138 (MQ=255)                                                                                             gTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                     <  2:30483/60‑1 (MQ=255)                                                                                             gTTATTTAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTc                                                                                                                     >  1:30483/1‑60 (MQ=255)                                                                                                 tttAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCat                                     <  2:165428/136‑1 (MQ=255)                                                                                                 tttAATGAACACTGGCAATCATTATGACGTTTACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCat                                     >  1:165428/1‑136 (MQ=255)                                                                                                                              tttACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGc                                             <  1:399915/99‑1 (MQ=255)                                                                                                                              tttACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGc                                             >  2:399915/1‑99 (MQ=255)                                                                                                                               ttACCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtt    >  2:164490/1‑139 (MQ=255)                                                                                                                                 aCCCCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACtttt  >  2:481340/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        TAGCATTTGCTCATTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTT  >  NZ_CP009273/1060529‑1060795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑