| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,312,468 | T→C | T69A (ACG→GCG) | trpB ← | tryptophan synthase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,312,468 | 0 | T | C | 100.0% | 28.5 / NA | 10 | T69A (ACG→GCG) | trpB | tryptophan synthase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/5); total (5/5) | |||||||||||
TACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCG > NZ_CP009273/1312366‑1312597 | tACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGt > 1:530466/1‑139 (MQ=255) ccTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGaa < 1:414080/139‑1 (MQ=255) ggTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTcagca > 1:30390/1‑126 (MQ=255) ggTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTcagca < 2:30390/126‑1 (MQ=255) cAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACg < 1:318239/57‑1 (MQ=255) cAGCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACg > 2:318239/1‑57 (MQ=255) gCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCa < 1:157956/116‑1 (MQ=255) gCGTGGTGTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCa > 2:157956/1‑116 (MQ=255) tggtgTTCGCCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg > 1:288950/1‑139 (MQ=255) tcgtgtTCGCCCCGGCTGTAATGTTCTTGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGATCCTGAAATTCAGGAGCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGgcg < 2:99564/137‑1 (MQ=255) | TACCCATCCGCTTCGCCAGCAACGCCTGCCCCAGCACCTGGTTAGTTTTATGCGCGCCGCCGTGCAGCAAATCTTCACGCTTGAGATACAGCGTGGTGTTCGTCCCGGCTGTAATGTTCTGGCATTTGGTCAGCGCGGTTGGACGCCCGGCATAGTTTTTCAGCAGGTCGTTGAACTGAGCCTGAAATTCAGGATCTTTTTGCGCACTGACAAAAGCTTCTTCCAGCTGGCG > NZ_CP009273/1312366‑1312597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |