Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,444,183	T→G	Q475P (CAG→CCG)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,444,183	0	T	G	100.0%	103.4 / NA	29	Q475P (CAG→CCG)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/14);  total (15/14)

CGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCAT  >  NZ_CP009273/1444049‑1444315                                                                                                                                       |                                                                                                                                       cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCggg                                                                                                                                      <  1:554168/137‑1 (MQ=255)         gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            <  2:287201/139‑1 (MQ=255)         gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                                            <  2:483936/139‑1 (MQ=255)            gCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          <  2:251347/138‑1 (MQ=255)                 ggCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTta                                                                                                                    >  1:6993/1‑139 (MQ=255)                  gCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTta                                                                                                                    >  1:356775/1‑138 (MQ=255)                  gCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTta                                                                                                                    >  1:5417/1‑138 (MQ=255)                                            aTGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCaaa                                                                                        >  1:247172/1‑138 (MQ=255)                                                                    gTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTcc                                                               <  2:356775/139‑1 (MQ=255)                                                                       aTAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCAt                                                             <  1:553828/138‑1 (MQ=255)                                                                               ccACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGc                                                     >  2:241373/1‑138 (MQ=255)                                                                                         gATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGcc                                                    >  1:258969/1‑129 (MQ=255)                                                                                         gATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGcc                                                    <  2:258969/129‑1 (MQ=255)                                                                                          aTCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCgg                                         >  1:465180/1‑139 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          <  1:368138/55‑1 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGa                                                                                                                          >  2:368138/1‑55 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTccgg                                                                                                            >  1:133273/1‑69 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTccgg                                                                                                            <  2:133273/69‑1 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAgcg                                                                           >  1:340181/1‑100 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAgcg                                                                           <  2:340181/100‑1 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                    >  2:446369/1‑139 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATg                                    >  2:169062/1‑139 (MQ=255)                                                                                                    ccacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGttt                                >  2:510858/1‑138 (MQ=255)                                                                                                    ccacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGttt                                >  1:16956/1‑138 (MQ=255)                                                                                                     cacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAACGCGAGGGATCTCCAGCGGCACGCCAGTGTAGTCGGCGATGGTTTCa                              <  1:169062/139‑1 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCgg                                                          <  1:135768/101‑1 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCgg                                                          >  2:135768/1‑101 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa                    <  2:154125/139‑1 (MQ=255)                                                                                                                              aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg     <  2:300401/139‑1 (MQ=255)                                                                                                                              aCGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg     <  2:247172/139‑1 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCAt  >  2:67031/1‑139 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAggg                                                                     <  2:365298/70‑1 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAggg                                                                     >  1:365298/1‑70 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg     >  2:452348/1‑134 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACgggg     <  1:452348/134‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAC‑‑GTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCACTGCGTTAGACGGGGCAT  >  NZ_CP009273/1444049‑1444315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑