Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,624,590	T→C	D194G (GAC→GGC)	ydfI ←	mannitol dehydrogenase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,624,590	0	T	C	100.0%	59.1 / NA	18	D194G (GAC→GGC)	ydfI	mannitol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/8);  total (10/8)

TGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGG  >  NZ_CP009273/1624481‑1624715                                                                                                              |                                                                                                                              tGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCAtg                                                                                                  >  1:113267/1‑139 (MQ=255)  gTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCAtgt                                                                                                 >  1:237418/1‑139 (MQ=255)  gTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCAtgt                                                                                                 >  2:517946/1‑139 (MQ=255)       tGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGc                                                                                                     >  1:413965/1‑130 (MQ=255)       tGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGc                                                                                                     <  2:413965/130‑1 (MQ=255)                       tCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTcaca                                                                                          <  1:443933/125‑1 (MQ=255)                       tCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTcaca                                                                                          >  2:443933/1‑125 (MQ=255)                                gttgATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGa                                                                    <  2:288746/138‑1 (MQ=255)                                 ttgATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAg                                                                  >  2:90574/1‑139 (MQ=255)                                      gggAAAGTCACGTTATCTTCGTTCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTg                                                             >  2:556685/1‑139 (MQ=255)                                      gggAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTg                                                             >  1:17135/1‑139 (MQ=255)                                                         cGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGcc                                            <  2:285151/137‑1 (MQ=255)                                                          gATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAg                                          <  2:554681/138‑1 (MQ=255)                                                           aTCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAg                                          <  2:279594/137‑1 (MQ=255)                                                           aTCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAg                                          >  1:279594/1‑137 (MQ=255)                                                                                tCAACGGCTTGTGCGTAGGAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTg                   <  2:17135/139‑1 (MQ=255)                                                                                                atgAATTGACAACGCCACTCATAACATGACCGTTTTCTGGCATGTTGTCACATTACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCTTCAACAATAACCCCTGTTGCTGTTTTCGTCtggtg   >  2:551245/3‑139 (MQ=255)                                                                                                 ggAAGTGACAACGCCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCtggtgg  <  2:113267/139‑1 (MQ=255)                                                                                                              |                                                                                                                              TGTCACTGCGGGCACAATACGGTCCACCATTGTTGATGGGAAAGTCACGTTATCTTCGATCCATTGTGCCAGTTTTACATCAACGGCTTGTGCGTAGGAAGTGACAACGTCACGCATAACATGACCGTTTTCTGGCATGTTGTCACATGACATGACGGTAAATGCGGGAAGTCCTGCCGCTTTACGGCGAGCCAGCGCCTCAACAATCACCCCTGTTGCTGTTTTCGGCTGGTGG  >  NZ_CP009273/1624481‑1624715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑