Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,679,189	A→G	N225S (AAC→AGC)	tus →	DNA replication terminus site‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,679,189	0	A	G	100.0%	111.9 / NA	30	N225S (AAC→AGC)	tus	DNA replication terminus site‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

GTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCG  >  NZ_CP009273/1679063‑1679320                                                                                                                               |                                                                                                                                    gTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTaa                                                                                                                          >  2:96940/1‑138 (MQ=255)  tgatgaAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTaaa                                                                                                                         >  1:202256/1‑138 (MQ=255)          tCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACg                                                                                                                <  2:482707/139‑1 (MQ=255)             tGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTcc                                                                                                             >  2:316747/1‑139 (MQ=255)                    gCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATAAAACGTCCGGCGAAg                                                                                                      >  2:295466/1‑139 (MQ=255)                        gAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGc                                                                                                  <  2:267198/139‑1 (MQ=255)                               gCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGAt                                                                                           >  1:18651/1‑139 (MQ=255)                                    aaaTCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGccc                                                                                      <  1:454425/139‑1 (MQ=255)                                         accacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                                 <  1:106229/139‑1 (MQ=255)                                                  tgtCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACa                                                                          >  2:275293/1‑137 (MQ=255)                                                    tCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAgg                                                                      <  2:202256/139‑1 (MQ=255)                                                          cGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATc                                                                 >  2:222605/1‑138 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTg                                                                               >  2:203788/1‑122 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTg                                                                               <  1:203788/122‑1 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                               >  1:485576/1‑138 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                               >  2:226340/1‑138 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                               >  1:239629/1‑138 (MQ=255)                                                            tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                               >  2:492483/1‑138 (MQ=255)                                                               aCGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAAc                                                           <  2:232422/139‑1 (MQ=255)                                                                             gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                              <  1:226340/138‑1 (MQ=255)                                                                             gCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                              <  1:222605/138‑1 (MQ=255)                                                                                 aGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGcc                                         <  1:492483/139‑1 (MQ=255)                                                                                          gAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTAcaccac                                <  2:527275/139‑1 (MQ=255)                                                                                            gCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGcc                                             >  1:367732/1‑124 (MQ=255)                                                                                            gCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGcc                                             <  2:367732/124‑1 (MQ=255)                                                                                               agagTATCAGGATATCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGAtt                           >  2:291678/1‑139 (MQ=255)                                                                                                           tatCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAAt               <  2:485576/139‑1 (MQ=255)                                                                                                             tCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                              >  1:154089/1‑106 (MQ=255)                                                                                                             tCGCTGCCCTGCCACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGc                                              <  2:154089/106‑1 (MQ=255)                                                                                                                        ccACAGAGCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgcg  <  1:96940/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                    GTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCG  >  NZ_CP009273/1679063‑1679320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑