Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→G	W30G (TGG→GGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	G	100.0%	161.3 / NA	45	W30G (TGG→GGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/23);  total (22/23)

TACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAAC  >  NZ_CP009273/49776‑50040                                                                                                                                       |                                                                                                                                   tACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCACGCCGTGGAACCTGCCTACCGATCTCGCCGGGt                                                                                                                                 <  1:487579/138‑1 (MQ=255)        tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACg                                                                                                                          >  2:277436/1‑138 (MQ=255)        tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACg                                                                                                                          >  2:283643/1‑138 (MQ=255)         aCGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACGc                                                                                                                         >  2:429330/1‑138 (MQ=255)          cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCa                                                                                                                        <  1:193380/138‑1 (MQ=255)                gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCa                                                                                                                        >  1:95366/1‑132 (MQ=255)                gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCa                                                                                                                        <  2:95366/132‑1 (MQ=255)                    cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                              >  2:480338/1‑138 (MQ=255)                    cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaa                                                                                                              >  1:500690/1‑138 (MQ=255)                     gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTtaaata                                                                                                            <  1:448220/139‑1 (MQ=255)                         aTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                                                                        <  1:230513/139‑1 (MQ=255)                         aTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                                                                        <  1:92008/139‑1 (MQ=255)                            ttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                                                                      <  2:12108/138‑1 (MQ=255)                                     gggAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                                                                                            >  1:217980/1‑139 (MQ=255)                                                      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGt                                                                                                                                 <  2:451177/85‑1 (MQ=255)                                                      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGt                                                                                                                                 >  1:451177/1‑85 (MQ=255)                                                      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                                                                          <  2:510153/108‑1 (MQ=255)                                                      aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTtaaataaa                                                                                                          >  1:510153/1‑108 (MQ=255)                                                                cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                                                                        <  1:294293/132‑1 (MQ=255)                                                                cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                                                                        >  2:294293/1‑132 (MQ=255)                                                                  gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                                                               >  2:97591/1‑139 (MQ=255)                                                                  gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                                                                      >  1:397553/1‑100 (MQ=255)                                                                  gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCg                                                                                                      <  2:397553/100‑1 (MQ=255)                                                                               aTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCaa                                                                                                                       >  2:474520/1‑70 (MQ=255)                                                                               aTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCaa                                                                                                                       <  1:474520/70‑1 (MQ=255)                                                                                  gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               <  1:277285/139‑1 (MQ=255)                                                                                   cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               >  2:546583/1‑138 (MQ=255)                                                                                   cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGGTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               >  1:262089/1‑138 (MQ=255)                                                                                      tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               <  1:32824/135‑1 (MQ=255)                                                                                      tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               >  2:32824/1‑135 (MQ=255)                                                                                        tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAata                                          <  1:97591/138‑1 (MQ=255)                                                                                        tCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAata                                          <  1:283643/138‑1 (MQ=255)                                                                                                    aCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCt                                   <  2:390611/133‑1 (MQ=255)                                                                                                    aCGCCATGCCGTGGAACCTGCCTACCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCt                                   >  1:390611/1‑133 (MQ=255)                                                                                                         aTGCCGTGGAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAc                        <  1:47731/139‑1 (MQ=255)                                                                                                         aTGCCGTGGAACCTGCCTGCCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAAc                        <  1:167097/139‑1 (MQ=255)                                                                                                                ggAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                                                                        >  2:326126/1‑52 (MQ=255)                                                                                                                ggAACCTGCCTGTCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAAcc                                                                                                        <  1:326126/52‑1 (MQ=255)                                                                                                                     ctgcctacCGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACg            <  2:65729/139‑1 (MQ=255)                                                                                                                      tgcctgtcGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCgg                     >  2:282953/1‑129 (MQ=255)                                                                                                                      tgcctgtcGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCgg                     <  1:282953/129‑1 (MQ=255)                                                                                                                      tgcctaccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGa           >  2:145246/1‑139 (MQ=255)                                                                                                                       gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               <  1:7706/102‑1 (MQ=255)                                                                                                                       gcctgccGATCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaa                                               >  2:7706/1‑102 (MQ=255)                                                                                                                               aTCTCGCCGGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAAc  >  2:120042/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                   TACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAAC  >  NZ_CP009273/49776‑50040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑