Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,747,881	A→C	G97G (GGT→GGG)	ydhV ←	aldehyde ferredoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,747,881	0	A	C	100.0%	80.4 / NA	22	G97G (GGT→GGG)	ydhV	aldehyde ferredoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/12);  total (10/12)

TGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCG  >  NZ_CP009273/1747757‑1747980                                                                                                                             |                                                                                                    tGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTaa                                                                                       <  1:399793/139‑1 (MQ=255)     gtcgtATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATggg                                                                                   >  2:393045/1‑139 (MQ=255)         tATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCgg                                                                               <  2:287345/139‑1 (MQ=255)                cGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCggannnn                                                                          >  2:283572/1‑132 (MQ=255)                cGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCa                                                                          >  1:1249/1‑137 (MQ=255)                   aTTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGAc                                                                     >  1:26595/1‑139 (MQ=255)                                       aaGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGa                                                 <  2:1249/139‑1 (MQ=255)                                                       cATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                                 <  1:191073/139‑1 (MQ=255)                                                        aTCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGc                                >  1:330205/1‑139 (MQ=255)                                                          cgacgaCTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCgg                              >  1:337756/1‑139 (MQ=255)                                                            acgacTAAATTTCCTTAGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTa                            <  2:330205/139‑1 (MQ=255)                                                                                 aaaGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTg        <  2:26595/138‑1 (MQ=255)                                                                                       gAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCg  <  1:393045/138‑1 (MQ=255)                                                                                       gAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCg  <  2:337756/138‑1 (MQ=255)                                                                                               ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGa                                                                                                >  1:509733/1‑36 (MQ=255)                                                                                               ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGa                                                                                                <  2:509733/36‑1 (MQ=255)                                                                                               ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTa                            >  1:546626/1‑104 (MQ=255)                                                                                               ggTGATATTTACGCGAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTa                            <  2:546626/104‑1 (MQ=255)                                                                                                          cgcgAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTcatacat               >  1:562158/1‑106 (MQ=255)                                                                                                          cgcgAGAACTACAGGGGGCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTcatacat               <  2:562158/106‑1 (MQ=255)                                                                                                                       gggggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                                 >  2:245204/1‑75 (MQ=255)                                                                                                                       gggggCCCCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTgg                                 <  1:245204/75‑1 (MQ=255)                                                                                                                             |                                                                                                    TGACGTCGTATCCAGCGAATTTCATTTGCGCTGCAAAAAAGCCACCCATATGGGCATCGACGACTAAATTTCCTTTGGTAAAAGTAGAAAGTGAGGTGATATTTACGCGAGAACTACAGGGGGCACCAGATCCAGTTAATGGGCCGGTAGCAAAGACTAATTTATTCGCTTCATCGAAAGGTTTCGTGCCTGGCGGTACTTCGTCATACATAATTTTGTAGCCG  >  NZ_CP009273/1747757‑1747980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑