Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,120	A→G	I157V (ATT→GTT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,120	0	A	G	100.0%	173.2 / NA	46	I157V (ATT→GTT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

CGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775986‑1776245                                                                                                                                       |                                                                                                                              cGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCt                                                                                                                           <  2:382304/139‑1 (MQ=255)              aCAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGtt                                                                                                              <  1:96090/139‑1 (MQ=255)                                cgcgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCcgc                                                                                            >  1:538134/1‑139 (MQ=255)                                  cgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCcgctc                                                                                          >  1:491844/1‑139 (MQ=255)                                                 gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           >  1:151545/1‑139 (MQ=255)                                                 gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           <  2:152239/139‑1 (MQ=255)                                                 gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           <  2:487663/139‑1 (MQ=255)                                                 gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           >  1:304413/1‑139 (MQ=255)                                                    ttCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGc                                                                        >  1:91809/1‑139 (MQ=255)                                                              cgcgCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  2:116681/139‑1 (MQ=255)                                                                            aaGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGt                                                <  1:203458/139‑1 (MQ=255)                                                                               gAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTgg                                             >  1:332077/1‑139 (MQ=255)                                                                               gAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTgg                                             >  1:389237/1‑139 (MQ=255)                                                                                        tCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTaaa                                      >  2:18248/1‑137 (MQ=255)                                                                                          aCTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAgg                                  >  2:534423/1‑139 (MQ=255)                                                                                                 tGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGca                                                            <  1:534280/106‑1 (MQ=255)                                                                                                 tGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGca                                                            >  2:534280/1‑106 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:151545/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:280185/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:332077/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:534423/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:304413/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:389237/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:18248/139‑1 (MQ=255)                                                                                                    aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:538134/139‑1 (MQ=255)                                                                                                      gCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:120767/137‑1 (MQ=255)                                                                                                      gCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        >  1:120767/1‑137 (MQ=255)                                                                                                        tGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              >  1:29248/1‑97 (MQ=255)                                                                                                        tGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  2:29248/97‑1 (MQ=255)                                                                                                          gggATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCacac                   >  1:426598/1‑138 (MQ=255)                                                                                                           ggATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCacac                   >  2:63070/1‑137 (MQ=255)                                                                                                            gatgatATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAg                <  1:259737/139‑1 (MQ=255)                                                                                                              tgatATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              <  2:212371/139‑1 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              >  2:284318/1‑89 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  1:284318/89‑1 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:150451/127‑1 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        >  1:150451/1‑127 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              >  1:336442/1‑137 (MQ=255)                                                                                                                atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAAc             <  2:426598/138‑1 (MQ=255)                                                                                                                       cGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              <  2:209343/130‑1 (MQ=255)                                                                                                                       cGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGaa              >  1:209343/1‑130 (MQ=255)                                                                                                                          aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:421868/139‑1 (MQ=255)                                                                                                                          aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:243880/139‑1 (MQ=255)                                                                                                                          aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:171860/139‑1 (MQ=255)                                                                                                                           gCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACg                                                    <  1:521495/88‑1 (MQ=255)                                                                                                                           gCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACg                                                    >  2:521495/1‑88 (MQ=255)                                                                                                                                       |                                                                                                                              CGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775986‑1776245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑