Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,806,054	T→C	S288S (AGT→AGC)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,806,054	0	T	C	100.0%	124.6 / NA	35	S288S (AGT→AGC)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/23);  total (12/23)

CCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCG  >  NZ_CP009273/1805927‑1806166                                                                                                                                |                                                                                                                 ccAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                       <  2:138488/139‑1 (MQ=255)   aaaGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACt                                                                                                     <  2:195372/139‑1 (MQ=255)     aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCg                                                                                                               <  1:209974/127‑1 (MQ=255)     aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCg                                                                                                               >  2:209974/1‑127 (MQ=255)     aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTAc                                                                                                      >  2:548458/1‑136 (MQ=255)     aGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTAc                                                                                                      <  1:548458/136‑1 (MQ=255)         gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                <  2:83181/138‑1 (MQ=255)             cAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGt                                                                                           <  2:458772/139‑1 (MQ=255)                   cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      <  1:289900/138‑1 (MQ=255)                   cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      <  2:566177/138‑1 (MQ=255)                   cTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      <  1:124780/138‑1 (MQ=255)                           cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTAt                                                                                         >  2:276885/1‑127 (MQ=255)                           cAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTAt                                                                                         <  1:276885/127‑1 (MQ=255)                                           tGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAg                                                             >  1:506203/1‑139 (MQ=255)                                             ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGcc                                                           >  2:539787/1‑139 (MQ=255)                                             ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                            <  1:343273/138‑1 (MQ=255)                                                         gTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgct                                                <  2:323412/138‑1 (MQ=255)                                                          tCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgctgc                                              <  2:63916/139‑1 (MQ=255)                                                            gcgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                            <  1:22903/139‑1 (MQ=255)                                                                          tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                            >  2:519969/1‑109 (MQ=255)                                                                          tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                            <  1:519969/109‑1 (MQ=255)                                                                          tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAt                              >  2:21910/1‑139 (MQ=255)                                                                            tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      <  1:360798/81‑1 (MQ=255)                                                                            tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      >  2:360798/1‑81 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAtgtg                           <  2:126524/138‑1 (MQ=255)                                                                              ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAtgtg                           >  1:126524/1‑138 (MQ=255)                                                                                           cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgcg             <  1:539787/139‑1 (MQ=255)                                                                                            aGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                            <  2:469185/107‑1 (MQ=255)                                                                                            aGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                            >  1:469185/1‑107 (MQ=255)                                                                                                      aTTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCg  <  1:552886/139‑1 (MQ=255)                                                                                                      aTTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCg  <  1:341192/139‑1 (MQ=255)                                                                                                                    tAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      >  2:356225/1‑41 (MQ=255)                                                                                                                    tAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGc                                                                                      <  1:356225/41‑1 (MQ=255)                                                                                                                         gCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                            >  2:36299/1‑78 (MQ=255)                                                                                                                         gCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                            <  1:36299/78‑1 (MQ=255)                                                                                                                                |                                                                                                                 CCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCG  >  NZ_CP009273/1805927‑1806166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑