Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,486	A→C	T455P (ACT→CCT)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,486	0	A	C	100.0%	84.4 / NA	23	T455P (ACT→CCT)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/11);  total (12/11)

TACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGAAGGCAATAAAGTTC  >  NZ_CP009273/1809368‑1809606                                                                                                                       |                                                                                                                         taCCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaa                                                                                                      >  1:267892/1‑139 (MQ=255)      aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaaccgaa                                                                                                 >  1:219791/1‑139 (MQ=255)      aTACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTAcgaaccgaa                                                                                                 >  1:475218/1‑139 (MQ=255)        acacAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                                               >  1:265595/1‑139 (MQ=255)           caAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGa                                                                                            <  1:305306/139‑1 (MQ=255)                 aGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACg                                                                                      <  2:219791/139‑1 (MQ=255)                        ttGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTg                                                                               >  2:4602/1‑139 (MQ=255)                           gtggGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGcc                                                                            <  2:475218/139‑1 (MQ=255)                                ccGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCcgcgc                                                                        >  2:401687/1‑138 (MQ=255)                                     tttCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAact                                                                  <  2:267892/139‑2 (MQ=255)                                                         aCCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACgcgg                                              <  1:2775/139‑1 (MQ=255)                                                                  ccTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGa                                     <  1:401687/139‑1 (MQ=255)                                                                     gCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                                               <  1:279371/78‑1 (MQ=255)                                                                     gCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACt                                                                                               >  2:279371/1‑78 (MQ=255)                                                                      cccTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                                   <  1:4602/137‑1 (MQ=255)                                                                      cccTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                                   <  2:265595/137‑1 (MQ=255)                                                                                 aTTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAgcg                      >  1:399439/1‑139 (MQ=255)                                                                                       aGCTTGACGGCATGCATCGCATGGGGATCGACCCTAACCCGGAGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGACGAAACGCGGCGGTATTGAATCATAACAGGAGCGCGTgg                 >  2:149403/1‑138 (MQ=255)                                                                                                   tgcatCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGtttt                                       <  1:427901/104‑1 (MQ=255)                                                                                                   tgcatCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGtttt                                       >  2:427901/1‑104 (MQ=255)                                                                                                     catCGCATGGGGATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGAAGGCAATAAAGTTc  >  1:196553/1‑139 (MQ=255)                                                                                                               ggATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                                   <  1:343695/96‑1 (MQ=255)                                                                                                               ggATCGACCCTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAAt                                   >  2:343695/1‑96 (MQ=255)                                                                                                                       |                                                                                                                         TACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGATCGACACTAACCCGGCGAATTACGAACCGAACTCGATTAACGATAACTGGCCGCGCGAAACACCGCCGGGGCCGAAACGCGGCGGTTTTGAATCATACCAGGAGCGCGTGGAAGGCAATAAAGTTC  >  NZ_CP009273/1809368‑1809606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑