Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,819,028	T→C	E56G (GAA→GGA)	ves ←	environmental stress‑induced protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,028	0	T	C	100.0%	51.8 / NA	15	E56G (GAA→GGA)	ves	environmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/6);  total (9/6)

GTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATT  >  NZ_CP009273/1818925‑1819161                                                                                                        |                                                                                                                                      gTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTcgcc                                                                                                    >  2:508473/1‑139 (MQ=255)          aaaGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgatggat                                                                                           <  2:4917/139‑1 (MQ=255)                   aGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                                                                                               >  1:432487/1‑126 (MQ=255)                   aGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                                                                                               <  2:432487/126‑1 (MQ=255)                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                                                                                               <  1:15205/104‑1 (MQ=255)                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                                                                                               >  2:15205/1‑104 (MQ=255)                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                            >  1:300968/1‑139 (MQ=255)                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                            >  1:72674/1‑139 (MQ=255)                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                            >  2:3156/1‑139 (MQ=255)                                              aaaGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGcc                                                       >  2:285890/1‑139 (MQ=255)                                                     tCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTggg                                                 >  2:460158/1‑138 (MQ=255)                                                         cgcTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGaa                                               <  1:285890/136‑1 (MQ=255)                                                                                             tCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCca        >  1:520190/1‑139 (MQ=255)                                                                                             tCACTATCCTTCCCATGCCGGGAAACAAAGAAAACACCCCTTCCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCGCGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCAGTGCGCca        <  2:129563/139‑1 (MQ=255)                                                                                                   tCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGAtt  <  1:460158/139‑1 (MQ=255)                                                                                                        |                                                                                                                                      GTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCACAGATT  >  NZ_CP009273/1818925‑1819161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑