Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,865,194	A→C	N185H (AAC→CAC)	cdgI →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,865,194	0	A	C	100.0%	127.7 / NA	34	N185H (AAC→CAC)	cdgI	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/15);  total (19/15)

ATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGT  >  NZ_CP009273/1865062‑1865318                                                                                                                                     |                                                                                                                             aTTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATcc                                                                                                                        >  1:248693/1‑139 (MQ=255)     tCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGa                                                                                                                    >  1:24755/1‑139 (MQ=255)                          gTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACgg                                                                                                >  2:216993/1‑138 (MQ=255)                           tACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACggg                                                                                               >  2:213160/1‑138 (MQ=255)                           tACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACggg                                                                                               >  1:22413/1‑138 (MQ=255)                                  aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                       >  1:81080/1‑139 (MQ=255)                                  aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                       >  2:452697/1‑139 (MQ=255)                                             gTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCt                                                                             >  1:361476/1‑138 (MQ=255)                                                      tgTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttt                                                                   >  2:234264/1‑139 (MQ=255)                                                          tctattttctatATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAg                                                               >  1:172523/1‑139 (MQ=255)                                                                            cGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcac                                             <  1:216993/139‑1 (MQ=255)                                                                            cGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcac                                             <  2:172523/139‑1 (MQ=255)                                                                                       ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                  <  2:81080/139‑1 (MQ=255)                                                                                            tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta                             <  2:24755/139‑1 (MQ=255)                                                                                                tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAAc                         <  2:361476/139‑1 (MQ=255)                                                                                                  tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca                                              >  1:120125/1‑116 (MQ=255)                                                                                                  tAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca                                              <  2:120125/116‑1 (MQ=255)                                                                                                      cTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGAc                   <  1:337991/139‑1 (MQ=255)                                                                                                        cGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                  <  1:338433/122‑1 (MQ=255)                                                                                                        cGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                  >  2:338433/1‑122 (MQ=255)                                                                                                          cTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt               >  2:435396/1‑139 (MQ=255)                                                                                                          cTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAtt               >  1:132451/1‑139 (MQ=255)                                                                                                                ctctTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcaca                                            <  1:328229/104‑1 (MQ=255)                                                                                                                ctctTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGcaca                                            >  2:328229/1‑104 (MQ=255)                                                                                                                   ttttGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCt                                                                                         >  1:139484/1‑56 (MQ=255)                                                                                                                   ttttGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCt                                                                                         <  2:139484/56‑1 (MQ=255)                                                                                                                     ttGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg      <  1:234264/137‑1 (MQ=255)                                                                                                                        ttATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtcgt  <  2:405729/138‑1 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGGTGGCGCTGATCCCTTTTTTAGTTTTTCCTCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg      >  1:119324/1‑129 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCt                                                  >  2:322996/1‑85 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCt                                                  <  1:322996/85‑1 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                  >  2:129897/1‑101 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                  <  1:129897/101‑1 (MQ=255)                                                                                                                             gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg      <  2:119324/129‑1 (MQ=255)                                                                                                                                     |                                                                                                                             ATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGT  >  NZ_CP009273/1865062‑1865318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑