Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,000,655	A→G	E340G (GAA→GGA)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,000,655	0	A	G	100.0%	72.8 / NA	21	E340G (GAA→GGA)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

CATCGCGCATGGGGCGCGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGG  >  NZ_CP009273/2000532‑2000782                                                                                                                            |                                                                                                                                cATCGCGCATGGGGCGCGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAAcc                                                                                                                  <  2:378439/139‑1 (MQ=255)    cgcgCATGGGGCGCGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTg                                                                                                               >  1:297312/1‑139 (MQ=255)                       cGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTa                                                                                             <  2:235005/138‑1 (MQ=255)                                              gTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGaaaa                                                                     >  2:364149/1‑139 (MQ=255)                                                         gAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCtt                                                          >  1:141763/1‑139 (MQ=255)                                                         gAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCtt                                                          <  1:288729/139‑1 (MQ=255)                                                           aGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCg                                                        <  1:322333/139‑1 (MQ=255)                                                           aGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCg                                                        <  2:297312/139‑1 (MQ=255)                                                            gCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCgg                                                       >  1:74187/1‑139 (MQ=255)                                                            gCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCAGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCgg                                                       >  2:61454/1‑139 (MQ=255)                                                              cGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTa                                                     <  1:364149/139‑1 (MQ=255)                                                                aTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTAtt                                                   >  1:501632/1‑139 (MQ=255)                                                                      tCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCg                                                                                                     <  2:476988/83‑1 (MQ=255)                                                                      tCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCg                                                                                                     >  1:476988/1‑83 (MQ=255)                                                                         aCGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGa                                          <  2:141763/139‑1 (MQ=255)                                                                               tGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTa                                    <  1:26228/139‑1 (MQ=255)                                                                                    cTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTg                               <  2:74187/139‑1 (MQ=255)                                                                                    cTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTg                               >  1:47206/1‑139 (MQ=255)                                                                                             aaTCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACg                      <  1:153209/139‑1 (MQ=255)                                                                                                            ccGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGgtggtg       <  2:425249/139‑1 (MQ=255)                                                                                                                  cAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACggg  >  2:549498/1‑138 (MQ=255)                                                                                                                            |                                                                                                                                CATCGCGCATGGGGCGCGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGG  >  NZ_CP009273/2000532‑2000782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑