Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,052,030	T→C	D433G (GAT→GGT)	yeeO ←	toxic metabolite efflux MATE transporter YeeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,052,030	0	T	C	100.0%	76.0 / NA	22	D433G (GAT→GGT)	yeeO	toxic metabolite efflux MATE transporter YeeO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

CATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACC  >  NZ_CP009273/2051894‑2052165                                                                                                                                         |                                                                                                                                        cATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGAccc                                                                                                                                       <  1:188483/139‑1 (MQ=255)  aTCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCt                                                                                                                                      <  2:131310/139‑1 (MQ=255)  aTCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACAACATGTTTAACCTGTGGACCCt                                                                                                                                      <  1:106718/139‑1 (MQ=255)                                    cACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAgg                                                                                                     >  2:417724/1‑138 (MQ=255)                                      ccTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAg                                                                                                  >  1:292622/1‑139 (MQ=255)                                                 aGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAg                                                                                       >  1:46832/1‑139 (MQ=255)                                                                       gACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAagag                                                                 >  1:305421/1‑139 (MQ=255)                                                                                  cATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGc                                                         >  1:17615/1‑136 (MQ=255)                                                                                          aaGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGaa                                                            >  1:280156/1‑125 (MQ=255)                                                                                          aaGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGaa                                                            <  2:280156/125‑1 (MQ=255)                                                                                                  aGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAAc                                       <  1:417724/138‑1 (MQ=255)                                                                                                                 acaACGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTgc                        <  2:305421/138‑1 (MQ=255)                                                                                                                   aaCGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTgcgc                      >  1:261212/1‑138 (MQ=255)                                                                                                                   aaCGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTgcgc                      >  2:302814/1‑138 (MQ=255)                                                                                                                     cGACATGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTAt                   <  2:17615/139‑1 (MQ=255)                                                                                                                          tGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGcc              >  2:300631/1‑139 (MQ=255)                                                                                                                          tGTTTAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGcc              >  2:317851/1‑139 (MQ=255)                                                                                                                            tttAACCTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGccc             <  2:228553/138‑1 (MQ=255)                                                                                                                                 ccTGTGGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTAcccc       <  2:46832/139‑1 (MQ=255)                                                                                                                                     tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGcc                                            >  2:239983/1‑98 (MQ=255)                                                                                                                                     tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGccc             <  2:271816/129‑1 (MQ=255)                                                                                                                                     tgGACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGccc             >  1:271816/1‑129 (MQ=255)                                                                                                                                      ggACCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAAcc  >  2:335323/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        CATCGAAACCCACATGGCGTAACGGGCATCACGAGCACCTTTAAATCCAGCGGGTAGCACCCATGAGGCGGACCAAATAGGCATAAATAAAGCATTTAGCCAAATCAGAATCACAACGACATGTTTAACCTGTGGATCCTGGGTGTAAAACGATGCCATAACCCCGGCAAAGGGAGCCGTTAGCCAGGCGATGGCCGTTAATCCAAGAGTGGAAAGCCAGAACACATGCCGCAACTGAATCTCTGCTTGCGCTATCTGCCCTACCCCCAACC  >  NZ_CP009273/2051894‑2052165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑