Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,065,124	T→G	G35G (GGT→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,065,124	0	T	G	100.0%	39.9 / NA	12	G35G (GGT→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/6);  total (6/6)

CACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2064996‑2065258                                                                                                                                 |                                                                                                                                       cACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTg                                                                                                                              >  1:380958/1‑139 (MQ=255)    ccTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCAc                                                                                                                           <  2:43855/139‑1 (MQ=255)              aGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGGGGCGGGTGCACCGGCCCTTGc                                                                                                                 >  2:310382/1‑139 (MQ=255)                         atgaAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAgtcacg                                                                                                       <  2:380958/138‑1 (MQ=255)                                                cTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACt                                                                                                                          >  1:520796/1‑96 (MQ=255)                                                cTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACt                                                                                                                          <  2:520796/96‑1 (MQ=255)                                                  aCAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGGCACGTCCCTCCCGGGGCTGGCTGCTGACAt                                                                             >  1:443839/1‑139 (MQ=255)                                                      gCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGAGGCGGGTGCCCTGTCCCTTGCCCCCGTCACCCCACTCCCCGGGCTGGCTGCTGACATCCtt                                                                         >  1:434533/1‑139 (MQ=255)                                                           tATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCa                                                                                                                            >  2:366774/1‑83 (MQ=255)                                                           tATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCa                                                                                                                            <  1:366774/83‑1 (MQ=255)                                                             tGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCAcc                                                                  <  1:310382/139‑1 (MQ=255)                                                                    acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGgag                                                            <  2:543723/138‑1 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACa                                                                              <  1:517426/63‑1 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACa                                                                              >  2:517426/1‑63 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTAc     >  1:174985/1‑136 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTAc     <  2:174985/136‑1 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  1:7068/1‑139 (MQ=255)                                                                                                                             gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  1:25851/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       CACCCTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2064996‑2065258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑