Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,071,153	G→C	G35A (GGC→GCC)	cbtA →	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,071,153	0	G	C	100.0%	63.9 / NA	18	G35A (GGC→GCC)	cbtA	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/8);  total (10/8)

TATATCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCG  >  NZ_CP009273/2071021‑2071283                                                                                                                                     |                                                                                                                                   tataTCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGACCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTca                                                                                                                               <  2:72268/138‑1 (MQ=255)         cATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTg                                                                                                                          <  1:536142/135‑1 (MQ=255)              cAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGacaca                                                                                                                 >  2:387335/1‑139 (MQ=255)                                gAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACgt                                                                                               >  1:538029/1‑139 (MQ=255)                                     cATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAt                                                                                          >  1:353886/1‑139 (MQ=255)                                          ccTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                                                                      <  2:432947/138‑1 (MQ=255)                                           cTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca                                                                                    <  2:353886/139‑1 (MQ=255)                                                                                                     aCTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGa                                                                                                   <  1:37233/66‑1 (MQ=255)                                                                                                     aCTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGa                                                                                                   >  2:37233/1‑66 (MQ=255)                                                                                                         ctgTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACgcgc                      >  1:354157/1‑139 (MQ=255)                                                                                                              ccGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGt                  <  2:426249/138‑1 (MQ=255)                                                                                                                 aCTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                                                              <  1:271088/91‑1 (MQ=255)                                                                                                                 aCTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                                                              >  2:271088/1‑91 (MQ=255)                                                                                                                 aCTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCg               >  1:73294/1‑138 (MQ=255)                                                                                                                   tgctgGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca                                                                   >  2:147724/1‑84 (MQ=255)                                                                                                                   tgctgGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca                                                                   <  1:147724/84‑1 (MQ=255)                                                                                                                        gACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGAc        >  1:107528/1‑138 (MQ=255)                                                                                                                             gCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCg  >  2:334098/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                   TATATCTGCATATCAGGAAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCG  >  NZ_CP009273/2071021‑2071283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑