Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	75,122	G→A	A339T (GCA→ACA)	setA →	sugar efflux transporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	75,122	0	G	A	100.0%	73.8 / NA	19	A339T (GCA→ACA)	setA	sugar efflux transporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

TAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCA  >  NZ_CP009273/75010‑75253                                                                                                                 |                                                                                                                                    tAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAAc                                                                                                              <  1:13983/136‑1 (MQ=255) tAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAAc                                                                                                              <  2:409373/136‑1 (MQ=255)         gTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                   <  1:418017/139‑1 (MQ=255)         gTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                   >  2:320824/1‑139 (MQ=255)                 ttGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTacc                                                                                                                             >  1:187559/1‑105 (MQ=255)                 ttGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTacc                                                                                                                             <  2:187559/105‑1 (MQ=255)                         gCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctggct                                                                                   >  2:336206/1‑139 (MQ=255)                             cAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                                                               <  2:82008/139‑1 (MQ=255)                                                   tCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGcac                                                          <  2:214114/138‑1 (MQ=255)                                                    cGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGcaca                                                         >  1:80936/1‑138 (MQ=255)                                                       cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                     >  1:147117/1‑139 (MQ=255)                                                       cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                     >  1:432669/1‑139 (MQ=255)                                                                 ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                           <  1:336206/139‑1 (MQ=255)                                                                      tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                      >  2:92933/1‑139 (MQ=255)                                                                        ggATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGt                                    <  1:445729/139‑1 (MQ=255)                                                                           tGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTa                                 <  2:184023/139‑1 (MQ=255)                                                                                     tCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAAt                        <  2:147117/138‑1 (MQ=255)                                                                                       aGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGc                     >  2:172387/1‑139 (MQ=255)                                                                                                          agCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCa  <  1:172387/139‑1 (MQ=255)                                                                                                                 |                                                                                                                                    TAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCA  >  NZ_CP009273/75010‑75253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑