Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,237,080	G→C	R18G (CGA→GGA)	folE ←	GTP cyclohydrolase I FolE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,237,080	0	G	C	100.0%	67.5 / NA	19	R18G (CGA→GGA)	folE	GTP cyclohydrolase I FolE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/8);  total (11/8)

TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC  >  NZ_CP009273/2236959‑2237198                                                                                                                          |                                                                                                                       tcatcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCAt                                                                                                       >  1:93404/1‑139 (MQ=255)           ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                             <  2:93404/139‑1 (MQ=255)           ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                             >  2:289222/1‑139 (MQ=255)           ggTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGc                                                                                             <  1:441997/139‑1 (MQ=255)                      agcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTAc                                                                                  >  1:218339/1‑139 (MQ=255)                       gcagcTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACt                                                                                 <  1:338975/139‑1 (MQ=255)                                        gTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTAt                                                                 >  1:120516/1‑138 (MQ=255)                                                aCCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTcc                                                        >  2:285915/1‑139 (MQ=255)                                                   aGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                      >  1:499163/1‑138 (MQ=255)                                                          aGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                               >  1:122548/1‑138 (MQ=255)                                                          aGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                               <  2:479104/138‑1 (MQ=255)                                                                                        tGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtta                >  1:289989/1‑139 (MQ=255)                                                                                               gcgggcgCAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCt         >  2:152667/1‑139 (MQ=255)                                                                                                     gcAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTa   <  1:285915/139‑1 (MQ=255)                                                                                                      cAGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAc  <  2:499163/139‑1 (MQ=255)                                                                                                       aGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGa                                             <  1:69115/95‑1 (MQ=255)                                                                                                       aGCGGTGTTTCCAGTCCTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGa                                             >  2:69115/1‑95 (MQ=255)                                                                                                                      ccTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                      >  1:40728/1‑71 (MQ=255)                                                                                                                      ccTCCCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                      <  2:40728/71‑1 (MQ=255)                                                                                                                          |                                                                                                                       TCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTAC  >  NZ_CP009273/2236959‑2237198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑