| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,243,084 | A→G | L5P (CTC→CCC) | yeiE ← | DNA‑binding transcriptional regulator YeiE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,243,084 | 0 | A | G | 95.8% | 79.9 / ‑6.5 | 24 | L5P (CTC→CCC) | yeiE | DNA‑binding transcriptional regulator YeiE |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (10/13); minor base T (0/1); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAA > NZ_CP009273/2242948‑2243219 | aaaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGgggg < 2:192774/139‑1 (MQ=255) cGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTCTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCa > 1:48519/1‑138 (MQ=255) cGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTCTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCAt < 2:48519/139‑1 (MQ=255) cccTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACAGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCtt < 2:880/137‑1 (MQ=255) gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg < 1:385748/108‑1 (MQ=255) gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTg > 2:385748/1‑108 (MQ=255) gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAt < 1:458348/110‑1 (MQ=255) gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAt > 2:458348/1‑110 (MQ=255) gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGat > 1:516073/1‑139 (MQ=255) cgcTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAt > 1:279211/1‑100 (MQ=255) cgcTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGAt < 2:279211/100‑1 (MQ=255) cgcTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATaaa > 2:563105/1‑139 (MQ=255) acaCGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATATAGATTAATTATAAATATATAATCAattttattt > 2:438193/3‑139 (MQ=255) ccGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCAt < 1:231302/73‑1 (MQ=255) ccGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCAt > 2:231302/1‑73 (MQ=255) ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGa > 2:194870/1‑80 (MQ=255) ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGa < 1:194870/80‑1 (MQ=255) ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc < 2:299034/139‑1 (MQ=255) ttGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc < 1:438193/139‑1 (MQ=255) tttCAATACTTCTGCAAAAACTTCCAACTGCCGgggg > 1:461410/1‑37 (MQ=255) tttCAATACTTCTGCAAAAACTTCCAACTGCCGgggg < 2:461410/37‑1 (MQ=255) tCAATACTTCTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGgag < 2:420342/138‑1 (MQ=255) cTGCAAACACTTCCAACTGCCGGGGGGTGATGTGCATAGt < 1:488666/40‑1 (MQ=255) cTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGt > 2:488666/1‑40 (MQ=255) cTGCAAAAACTTCCAACTGCCGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATg < 2:516073/138‑1 (MQ=255) ccAACTGCCGGTGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCAcc < 2:105643/138‑1 (MQ=255) cGGGGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGaaa < 1:320230/139‑1 (MQ=255) | AAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAA > NZ_CP009273/2242948‑2243219 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |