| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,351,416 | A→G | pseudogene (134/198 nt) | BW25113_RS11725 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,351,416 | 0 | A | G | 75.0% | 16.0 / 4.5 | 12 | pseudogene (134/198 nt) | BW25113_RS11725 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (4/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
TTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAACACAGGCCCACAGCCGATCCCCATGGGCCTTTGATATCAATAAC > NZ_CP009273/2351302‑2351528 | ttGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCAc > 1:425574/1‑139 (MQ=255) cGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAg > 1:540320/1‑139 (MQ=255) aggaaggaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGa < 1:390380/139‑1 (MQ=255) gaaggaCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATc > 2:258126/1‑139 (MQ=255) aCATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCa < 1:258126/139‑1 (MQ=255) aGGTTGGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAACACAGGCCCACAGc < 2:425574/139‑1 (MQ=255) aCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCa > 1:557250/1‑105 (MQ=255) aCAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCa < 2:557250/105‑1 (MQ=255) ttGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAACACAGGCCCACAGCCGATCCCCATGGGCCTTTGATATCAATAAc > 1:516299/1‑138 (MQ=255) tGCGCATGCTGGAACAGGGCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAACACAGGCCCACAGCCGATCCCCATGGGCCTTTGATATCAATAAc < 1:201892/137‑1 (MQ=255) ggCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAAcaca > 1:187455/1‑80 (MQ=255) ggCTTTGGTCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAAcaca < 2:187455/80‑1 (MQ=255) | TTGCTGAACGGCTGCGGCAGGAAGGACATCAAATTGGCTGGCAGGAAGGTAAATTAGAAGGTTTGCATGAACAAGCCATTAAAATTGCTTTGCGCATGCTGGAACAGGGCTTTGATCGTGACCAGGTGCTCGCGGCCACCCAGCTAAGCGAAGCCGATCTGGCAGCGAATAACCACTAATTAACACAGGCCCACAGCCGATCCCCATGGGCCTTTGATATCAATAAC > NZ_CP009273/2351302‑2351528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |